Highly stable surface plasmon resonance plates, microarrays, and methods
AssigneeLumera Corporation
InventorsNick Wolf, Danliang Jin, Anna M. Barklund, Raluca Dinu
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Abstract
An article, process, and method for surface plasmon resonance plates are described. A substrate is covered with a thin metal film onto which a second thin metal film is deposited. The surface of the second thin metal film is converted to the metal oxide which is used to covalently bond organosilanes to the surface. Reactive organosilanes containing terminal bonding groups are arranged in a plurality of spots that are surrounded by inert organosilanes. Biomolecule attachment to the binding group is detected or measured from surface plasmon signals from the first thin metal film.
Claims45
An article, comprising:
- a) a substrate;
- b) a first metal film overlying the substrate;
- c) a second thin metal film overlying the first metal film, wherein the surface of the second metal film comprises metal-oxygen bonds;
- and d) a reactive organosilane covalently bonded to the upper surface of the second thin metal film through metal-oxygen-silicon bonds.
A process, comprising:
- a) depositing a first metal film on a substrate;
- b) depositing a second thin metal film on the first metal film;
- c) converting the upper surface of the second thin metal film to the corresponding metal oxide;
- and d) reacting a functionalized organosilane with the metal oxide, whereby a reactive organosilane is formed on the upper surface of the second thin metal film.
A process comprising:
- a) depositing a first metal film on a substrate;
- b) depositing a second thin metal film on the first metal film;
- c) converting the upper surface of the second thin metal film to the corresponding metal oxide;
- d) reacting an inert organosilane with the metal oxide;
- e) patterning the inert organosilane to give a pattern of a plurality of metal oxide spots surrounded by inert organosilane;
- and f) reacting a functionalized organosilane with the metal oxide, whereby a reactive organosilane is formed in the metal oxide spots on the second thin metal film.
A method of detecting a biomolecule, comprising a) providing an article including:
- i) a substrate;
- ii) a first metal film overlying the substrate;
- iii) a second thin metal film overlying the first metal film;
- iv) an organosilane covalently bonded to the exposed upper surface of the second thin metal film through metal-oxygen-silicon bonds;
- and v) a binding group attached to the organosilane;
- and b) determining whether or not the biomolecule is bound to the binding group by detecting or measuring a surface plasmon resonance signal generated from the first metal film.
Dependencies are inferred from the claim text.