Field-switch sequencing
AssigneePacific Biosciences of California, Inc.
InventorsJohn G. K. Williams, Jon P. Anderson
Life of this patent
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Abstract
The present invention provides novel compositions, methods and apparatus for DNA sequencing that can be performed, e.g., in a two-electrode chamber. The present invention also provides a method for sequencing a nucleic acid comprising immobilizing a plurality of complexes comprising a target nucleic acid, a primer nucleic acid, and a polymerase onto a surface, contacting the surface with a plurality of charged particles comprising a nucleotide phosphate by applying an electric field, reversing the electric field to transport unbound charged particles away from the surface, and detecting the incorporation of a nucleotide phosphate into a single molecule of the primer nucleic acid.
Claims18
A method for sequencing a nucleic acid, said method comprising:
- (a) immobilizing a plurality of complexes comprising a target nucleic acid, a primer nucleic acid, and a polymerase onto a surface;
- (b) applying an electric field to contact said surface with a plurality of charged nucleic acid nanoparticles comprising nucleotide phosphate (NP);
- (c) removing said nucleotide phosphate (NP) from said charged nucleic acid nanoparticle, wherein said nucleotide phosphate is a substrate for said polymerase;
- (d) reversing said electric field to transport unbound charged nucleic acid nanoparticles away from the surface;
- and
- (e) detecting the incorporation of said nucleotide phosphate into a single molecule of the primer nucleic acid.
Dependencies are inferred from the claim text.