Inverted orientation for a microplate
AssigneeApplied Biosystems, LLC
InventorIan A. Harding
Life of this patent
Estimating the term…
Abstract
An inverted microplate for conducting a thermocycled amplification reaction of polynucleotide. The microplate can comprise a main body having a first and second surfaces and a plurality of wells disposed in the first surface. Each of the plurality of wells can comprise a well opening and a well bottom and be sized to receive an assay. A sealing cover can be operably coupled to the first surface of the main body to seal the well openings of the plurality of wells when the main body is inverted so that the assay is in contact with the sealing covering cover.
Claims92
A microplate for conducting a thermocycled amplification reaction of polynucleotide, said microplate comprising:
- a main body having a first and second surfaces;
- a plurality of wells disposed in said first surface, each of said plurality of wells having a well opening and a well bottom and sized to receive an assay;
- and
- a sealing cover operably coupled to said first surface of said main body, said sealing cover substantially sealing said well openings of said plurality of wells contain said assay and said main body is inverted so that said assay is in contact with said sealing cover.
A microplate for conducting a thermocycled amplification reaction of polynucleotide in a liquid sample, said microplate comprising:
- a main body having first and second surfaces;
- a plurality of wells disposed in said first surface, each of said plurality of wells having a well opening and a well bottom, each of said plurality of wells being sized to receive the liquid sample;
- and
- a cover having a cover surface, said cover surface being disposed adjacent to said first surface of said main body to cover said well opening of each of said plurality of wells,
- wherein said main body is oriented so that said liquid is in contact with said cover surface.
A method for performing thermocycling amplification of a liquid polynucleotide sample, said method comprising:
- (a) loading a quantity of said liquid polynucleotide sample into a microplate, said microplate having a main body having a first and second surfaces, and a plurality of wells disposed in said first surface, each of said plurality of wells having a well opening and a well bottom, each of said plurality of wells being sized to receive the liquid polynucleotide sample;
- (b) covering said main body with a cover, a surface of said cover being disposed adjacent to said first surface of said microplate so as to cover said well openings of said plurality of wells;
- (c) inverting said main body such that said liquid polynucleotide sample contacts said surface of said cover;
- and
- (d) thermocycling said main body to effect amplification of the polynucleotides in said liquid polynucleotide sample.
A method of conducting amplification comprising:
- (a) loading a liquid polynucleotide sample into a well of a microplate;
- (b) covering said well of said microplate with a cover such that said cover fluidly seals said liquid polynucleotide sample within said well;
- (c) inverting said microplate such that said liquid polynucleotide sample contacts said cover;
- and
- (d) thermocycling said microplate to promote amplification of polynucleotides in said liquid polynucleotide sample.
A method of conducting polymerase chain reaction analysis comprising:
- (a) loading an assay into a well of a microplate;
- (b) applying a cover to said well of said microplate such that said cover fluidly seals said assay within said well;
- (c) inverting said microplate such that said assay contacts said cover;
- and
- (d) thermocycling said microplate to promote amplification of said assay.
A microplate comprising:
- a main body;
- a plurality of wells disposed in said main body, said plurality of wells being sized to receive an assay;
- and
- a cover operably coupled to said main body, said cover substantially sealing at least one of said plurality of wells when said plurality of wells contain said assay and said main body is inverted so that said assay is in contact with said sealing covering cover.
Dependencies are inferred from the claim text.