Process for the observation of at least one sample region with a light raster microscope

Process for the observation of at least one sample region with a light raster microscope having an optical axis. comprising the steps of:

• (a) generating illumination light for illuminating a sample,
• (b) moving the illumination light and the sample relative to each other along at least one scanning axis substantially perpendicular to the optical axis, using a first scanner, the relative movement of the illumination light and the sample defining a plane of relative movement,
• (c) moving a second scanner at an angle to the plane of relative movement,
• (d) acquiring an image by coupling the movements of the first and second scanners and performing a three-dimensional sampling movement by illumination into an illumination region of the sample, and
• (e) calibrating at least the second scanner with the aid of a precalibration to at least one of the mass of the sample used and the mass of the objective used and the mass of the sample tray used for the generation of a control curve, and
• wherein step (c) is carried out with the aid of an acquired calibration curve, and wherein the sample is illuminated via scanners independent of one another, where the different scanners illuminate at least one of the same and different sample regions and there is at least one of an independent and common detection of the sample light.

Process for the observation of at least one sample region with a light raster microscope having an optical axis, comprising the steps of:

• (a) generating illumination light for illuminating a sample,
• (b) moving the illumination light arid the sample relative to each other along at least one scanning axis substantially perpendicular to the optical axis, using a first scanner, the relative movement of the illumination light and the sample defining a plane of relative movement,
• (c) moving a second scanner at an angle to the plane of relative movement,
• (d) acquiring an image by coupling the movements of the first and second scanners and performing a three-dimensional sampling movement by illumination into an illumination region of the sample, and
• (e) calibrating at least the second scanner with the aid of a precalibration to at least one of the mass of the sample used and the mass of the objective used and the mass of the sample tray used for the generation of a control curve, and
• wherein step (c) is carried out with the aid of an acquired calibration curve, and wherein the at least one first and second scanners are provided at least partially in duplicate in a first and second system which are directed at least one of simultaneously and sequentially onto the same samples.