Methods for generating resistance against CGMMV in plants
AssigneeKeygene N.V.
InventorsBernarda Gerharda Johanna Onstenk E. V. Fierens, Michiel Theodoor Jan De Both
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Abstract
The present invention relates to methods for generating resistance against Cucumber Green Mottle Mosaic Virus (CGMMV) in plants, in particular in plants susceptible to infection by CGMMV, such as Cucurbitaceae species, including melon, cucumber, watermelon and bottlegourd. The methods are based on the use of genetic constructs that induce post-transcriptional gene silencing and/or use a nucleotide sequence that encodes a defective variant of the replicase of CGMMV.
Claims17
A method for generating resistance in a plant or in a plant cell against infection with Cucumber Green Mottle Mosaic Virus (CGMMV), comprising generating a transformed plant or plant cell by transforming a plant or plant cell with one or more polynucleotide sequences that
- upon transcription into RNA generate resistance against infection with CGMMV in said plant and produce a sense and an antisense RNA molecule capable of forming a double stranded RNA region by base-pairing between the regions which are complementary, said polynucleotide sequences comprising a first and a second DNA sequence, wherein:
- said first DNA sequence comprises a promoter, operably linked to a first DNA region capable of being transcribed into a sense RNA molecule with a nucleotide sequence comprising a sense nucleotide sequence of at least 100 consecutive nucleotides having 100% sequence identity with the replicase encoding nucleotide sequence of the genome of the CGMMV virus capable of infecting the plant or the plant cell;
- said first DNA sequence optionally further comprises a DNA region involved in transcription termination and polyadenylation functioning in plant cells operably linked to said first DNA region;
- and
- said second DNA sequence comprises a second DNA region capable of being transcribed into an antisense RNA molecule with a nucleotide sequence comprising an antisense nucleotide sequence including at least 100 consecutive nucleotides, having 100% sequence identity with the complement of said at least 100 consecutive nucleotides of the sense nucleotide sequence;
- said second DNA sequence optionally further comprises a promoter operably linked to said second DNA region and optionally a DNA region involved in transcription termination and polyadenylation functioning in plant cells operably linked to said second DNA region,
- and wherein said replicase encoding nucleotide sequence of the genome of the CGMMV virus is:
- (A) the nucleotide sequence of SEQ ID NO:1;
- (B) the nucleotide sequence of SEQ ID NO:5;
- or
- (C) the nucleotide sequence of SEQ ID NO:3.
Dependencies are inferred from the claim text.