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US 7,459,600 B2Granted
Isolation, selection and propagation of animal transgenic stem cells
AssigneeThe University of Edinburgh
InventorsAustin Gerard Smith, Peter Scott Mountford
Life of this patent
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Abstract
Animal stem cells are obtained and maintained by culturing cells containing, in the genome, a selectable marker. Differential expression of the selectable marker enables preferential survival and/or division of the desired stem cells compared to the non-stem cells.
Claims12
A method of preparing a transgenic non-human mammal comprising:
- a) obtaining a desired stem cell by:
- i) culturing non-human mammalian embryonic stem (ES) cells containing a nucleic acid construct including a selectable marker operably linked to a promoter that preferentially expresses the selectable marker in mammalian ES cells;
- and
- ii) isolating mammalian ES cells expressing the selectable marker;
- and
- b) generating the transgenic non-human mammal therefrom.
3 more elements
A method of preparing a transgenic non-human mammal, said mammal comprising a selectable marker operatively linked to a promoter that preferentially expresses the selectable marker in mammalian ES cells, the method comprising:
- a) providing a blastocyst;
- b) providing a non-human mammalian ES cell comprising at least one nucleic acid construct encoding a selectable marker operably linked to a promoter which preferentially expresses said marker in a mammalian ES cell;
- c) introducing the mammalian ES cell into the blastocyst;
- d) transferring the blastocyst to a recipient;
- and
- e) allowing an embryo to develop to a chimeric mammal to enable germline transmission of the selectable marker.
3 more elements
Dependencies are inferred from the claim text.