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AssigneeNational Institute of Agrobiological Sciences
InventorsToshiki Tamura, Hideki Sezutsu, Isao Kobayashi, Katsura Kojima, Toshio Kanda, Keiro Uchino
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Transgenic silkworms comprising GFP whose expression is regulated by the sericin gene promoter were produced. Observation of the silk glands of the last instar larvae of the silkworms showed fluorescence only in the middle silk glands. GFP was secreted from middle silkgland cells from around the spinning stage, indicating that GFP moved into the gland lumen. Finally, GFP was spun into cocoon filaments, and cocoons containing large amounts of GFP were produced. Thus, by using the promoter region of the sericin gene, recombinant proteins can be produced in the middle silk glands. Furthermore, the recombinant proteins produced in the middle silk glands were readily secreted into the lumen of the middle silk glands.
A method for producing a protein of interest, wherein the method comprises the steps of:
A method for producing a protein of interest, wherein the method comprises the steps of:
A method for producing a transgenic silkworm that secretes a protein of interest into its cocoon filaments, wherein the method comprises the step of providing a silkworm egg comprising the following DNAs:
A method for producing a transgenic silkworm that secretes a protein of interest into its cocoon filaments, wherein the method comprises the step of providing a silkworm egg which is produced by crossing the following transgenic silkworms:
A transgenic silkworm whose genome comprises the following DNAs:
A method for expressing a protein of interest in the middle silkgland cells, wherein the method comprises the steps of providing a transgenic silkworm whose genome comprises the following DNAs:
A method for expressing a protein of interest in the middle silkgland cells, wherein the method comprises the steps of providing a transgenic silkworm which is produced by crossing the following transgenic silkworms:
A method for producing a transgenic silkworm that expresses a protein of interest in the middle silkgland cells, wherein the method comprises the step of providing a silkworm egg comprising the following DNAs:
A method for producing a transgenic silkworm that expresses a protein of interest in the middle silkgland cells, wherein the method comprises the step of providing a silkworm egg which is produced by crossing the following transgenic silkworms:
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