Chemically synthesized artificial promoter for high level expression of transgenes and a method for its synthesis

A promoter comprising at least one of each of the following elements, or functional fragments thereof, in the 5′ to 3′ direction:

• (i) domain II which comprises subdomain II(a), wherein subdomain II(a) is SEQ ID NO:
• 7;
• (ii) domain I, which comprises subdomain I(a), wherein subdomain I (a) is SEQ ID NO:
• 18, or subdomain I(a) is a functional sequence with at least 75% sequence identity to SEQ ID NO:
• 18;
• (iii) minimal domain (b), wherein minimal domain (b) is SEQ ID NO:
• 5;
• (iv) minimal domain (a), wherein minimal domain (a) is SEQ ID NO:
• 2;
• (v) region between minimal promoter (a) and a transcription start site context, wherein said region between minimal promoter (a) and said transcription start site context is SEQ ID NO:
• 12;
• (vi) transcription start site context, wherein said transcription start site context is SEQ ID NO:
• 4;
• (vii) 5′ untranslated leader region, wherein said 5′ untranslated leader region is SEQ ID NO:
• 13;
• (viii) translational initiation codon context, wherein said translational initiation codon context is SEQ ID NO:
• 14;
• and
• (ix) a polynucleotide encoding the amino acid sequence set forth in SEQ ID NO. 16.