Separation of albumin from protein samples
AssigneeThe Florida State University Research Foundation, Inc.
InventorsQing-Xiang (Amy) Sang, Ziad Joseph Sahab
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Abstract
The present invention generally relates to methods for separating albumin from samples comprising albumin and other proteins based on the pI of albumin. More particularly, the methods relate to forming an aqueous combination comprising the sample and an anion or a cation exchange material at various pHs, agitating the combination, and separating a fraction of unbound proteins from a concentrated solids fraction comprising the ion exchange material and bound proteins.
Claims35
A method for separating albumin from a sample comprising albumin and other proteins, the method comprising:
- combining the sample with an ion exchange material to form a first aqueous combination, the pH of the first aqueous combination being (i) less than the pI of a first fraction of the proteins in the first aqueous combination, (ii) greater than the pI of a second fraction of the proteins in the first aqueous combination, and (iii) a pH at which albumin precipitates from the first aqueous combination;
- agitating the first aqueous combination to induce the albumin to precipitate and to cause protein comprised by one of said first and second fractions to bind to the ion exchange material;
- and
- separating the ion exchange material from the agitated aqueous combination.
Dependencies are inferred from the claim text.