Fibroblast growth factor-like polypeptides
AssigneeAmgen Inc.
InventorsArlen Thomason, Benxian Liu, Dimitry Michael Danilenko
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Abstract
The present invention provides novel Fibroblast Growth Factor-like (FGF-like) polypeptides and nucleic acid molecules encoding the same. The invention also provides vectors, host cells, antibodies and methods for producing FGF-like polypeptides. Also provided for are methods for the diagnosis and treatment of diseases associated with FGF-like polypeptides.
Claims34
A polypeptide produced by a process comprising:
- (a) culturing a host cell containing a vector comprising a nucleic acid molecule having a nucleotide sequence:
- (i) as set forth in SEQ ID NO:
- 1;
- (ii) of the DNA insert in ATCC Deposit No. PTA-626;
- (iii) encoding the polypeptide of SEQ ID NO:
- 2;
- (iv) encoding the polypeptide of SEQ ID NO:
- 5;
- or
- (v) encoding the polypeptide of SEQ ID NO:
- 5 and an amino-terminal methionine;
- under conditions suitable to express the polypeptide;
- and optionally
- (b) isolating the polypeptide from the culture.
An isolated polypeptide comprising:
- (a) the amino acid sequence set forth in SEQ ID NO:
- 2;
- or
- (b) an amino acid sequence encoded by the DNA insert of ATCC Deposit No. PTA-626.
An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 5, optionally further comprising an amino-terminal methionine.
An isolated polypeptide encoded by a nucleic acid molecule comprising a nucleotide sequence:
- (a) as set forth in SEQ ID NO:
- 1;
- (b) of the DNA insert in ATCC Deposit No. PTA-626;
- (c) encoding the polypeptide of SEQ ID NO:
- 2;
- (d) encoding the polypeptide of SEQ ID NO:
- 5;
- or
- (e) encoding the polypeptide of SEQ ID NO:
- 5 and an amino-terminal methionine.
A process for preparing a chemically modified FGF-21 polypeptide, comprising:
- (a) reacting a polypeptide comprising:
- (i) the amino acid sequence of SEQ ID NO:
- 2;
- (ii) the amino acid sequence of SEQ ID NO:
- 5;
- or
- (iii) the amino acid sequence of SEQ ID NO:
- 5 further comprising an amino-terminal methionine;
- with polyethylene glycol (PEG) under conditions whereby the polypeptide becomes attached to at least one PEG group;
- and
- (b) obtaining the reaction products.
Dependencies are inferred from the claim text.