Factor VIII glycoforms
AssigneeLot 41 Acquisition Foundation, LLC
InventorsMyung-Sam Cho, Sham-Yuen Chan
Life of this patent
Estimating the term…
Abstract
The invention concerns glycosylated proteins having human factor VIII activity. In a preferred embodiment, the protein is glycosylated with oligosaccharides that include an alpha-(2,6)-linked sialic acid and a bisecting GlcNAc linked to a core beta-mannose.
Claims29
An isolated glycosylated protein having factor VIII procoagulant activity comprising the amino acid sequence of SEQ ID NO:
- 1 and an N-linked oligosaccharide having an alpha-(2,6)-linked sialic acid.
An isolated glycosylated protein having factor VIII procoagulant activity comprising the amino acid sequence of SEQ ID NO:
- 1 and a bisecting GlcNAc linked to a core beta-mannose.
An isolated protein having factor VIII procoagulant activity, wherein the amino acid sequence of the protein is at least 95% identical to SEQ ID NO:
- 1 and the protein further comprises a N-linked oligosaccharide comprising alpha-(2,6)-linked sialic acid.
An isolated protein having factor VIII procoagulant activity, wherein the amino acid sequence of the protein is at least 95% identical to SEQ ID NO:
- 1 and the protein further comprises a N-linked oligosaccharide comprising a bisecting GlcNAc linked to a core beta-mannose.
An isolated protein having Factor VIII procoagulant activity, wherein the amino acid sequence of the protein and the amino acid sequence of SEQ ID NO:
- 1 have at least 95% identity and the protein comprises an N-linked oligosaccharide comprising alpha-(2,6)-linked sialic acid, the isolated protein characterized in that at least 75% of the total protein matter present is protein having factor VIII procoagulant activity.
Dependencies are inferred from the claim text.