Recombinant gelatin-like proteins for use as plasma expanders
AssigneeFuji Film Manufacturing Europe B.V.
InventorsJan Bastiaan Bouwstra, Yuzo Toda
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Abstract
The invention relates to compositions suitable for plasma substitution comprising as a plasma expander a recombinant gelatin-like protein. Characteristic is that the gelatin-like protein essentially is free of hydroxyproline. This absence of hydroxyproline prevents the composition from gelling and thus allows the use of high-molecular weight proteins in order to establish a suitable colloid osmotic pressure. Specific advantage of the gelatin-like proteins is that these avoid the risk of anaphylactic shock that exists in conjunction with the use of commercially available preparations.
Claims20
A composition suitable as a substitute for plasma comprising a solution of saline in a physiologically acceptable concentration and a protein having a colloid osmotic function wherein the protein having a colloid osmotic function is a recombinant gelatin-like protein consisting essentially of repeats of Gly-Xaa-Yaa triplets and comprising a molecular weight of between 10,000 and 80,000 Daltons and at least one stretch of at least 30 consecutive Gly-Xaa-Yaa triplets, wherein at least 5% of the total number of protein amino acid residues in the gelatin-like protein are proline residues, wherein the gelatin-like protein is free from globular domains and does not comprise stretches of more than 20 amino acids without a proline residue wherein less than 2% of the amino acid residues, optionally less than 1%, in the gelatin-like protein are hydroxyproline residues and wherein said recombinant gelatin-like protein is present in the range of from 2 percent to 8 percent by weight of the composition.
A method of making a plasma expander composition wherein a recombinant gelatin-like protein is present in the range of from 2 percent to 8 percent by weight of the composition, the method comprising adding to a saline solution a recombinant gelatin-like protein consisting essentially of repeats of Gly-Xaa-Yaa triplets and comprising a molecular weight of between 10,000 and 80,000 Daltons and at least one stretch of at least 30 consecutive Gly-Xaa-Yaa triplets, wherein at least 5% of the total number of protein amino acid residues in the gelatin-like protein are proline residues, wherein the gelatin-like protein is free from globular domains and does not comprise stretches of more than 20 amino acids without a proline residue and wherein less than 2% of the amino acid residues in the gelatin-like protein are hydroxyproline residues.
A composition suitable as a substitute for plasma comprising a solution of saline in a physiologically acceptable concentration and a protein having a colloid osmotic function wherein the protein is selected from the group consisting of Biogel I, Biogel II and Biogel III and wherein the protein is present in the range of from 2 percent to 8 percent by weight of the composition.
A method of making a plasma expander composition comprising adding to a saline solution from 2 percent to 8 percent by weight of the composition of a protein selected from the group consisting of Biogel I, Biogel II and Biogel III.
Dependencies are inferred from the claim text.