Reagents for protection of peptide/proteins carbamylation in urea solutions utilizing non-ethylene-diamine like compounds
AssigneeN.V. Organon
InventorsMin Wan, Phillip Ropp
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Abstract
The present invention generally relates to non-ethylene diamine like compounds that prevent and/or delay carbamylation of peptides.
Claims13
A process for inhibiting and/or delaying carbamylation of a polypeptide in a urea and/or cyanate containing solution, the process comprising a step of adding a carbamylation-inhibiting compound to the solution, wherein said carbamylation-inhibiting compound is glycinamide.
A process for inhibiting and/or delaying carbamylation of a polypeptide in a urea and/or cyanate containing solution, the process comprising a step of adding a carbamylation-inhibiting compound to the solution, wherein the carbamylation-inhibiting compound is a dipeptide selected from the group consisting of Glycine-Glycine (Gly-Gly) and Glycine-Histidine (Gly-His).
A process for inhibiting and/or delaying carbamylation of a polypeptide in a urea and/or cyanate containing solution, the process comprising a step of adding a carbamylation-inhibiting compound selected from the group consisting of histidine and 4-hydroxyl proline to the solution, wherein the carbamylation-inhibiting compound is added to the solution in an amount effective to provide about 100% carbamylation protection of the polypeptide for a period of three weeks.
Dependencies are inferred from the claim text.