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US 7,459,294 B2Granted
Method of producing a compound by fermentation
AssigneeKosan Biosciences, Inc.
InventorsTimothy A. Leaf, Ruchir P. Desai, Peter Licari, Elaine Jennifer Woo
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Abstract
During the production of a product compound by fermentation, the concentration of a precursor compound is maintained within a pre-selected concentration range by having an adsorbent resin in contact with the culture medium. The adsorbent resin reversibly adsorbs precursor compound and, as un-adsorbed precursor compound is converted to product compound, adsorbed precursor compound is released from the resin, thus maintaining the concentration of precursor compound within the pre-selected range.
Claims12
A method of making a polyketide, comprising the steps of:
- (a) providing a culture medium containing (i) a precursor compound for said polyketide, said precursor compound being selected from the group consisting of a starter unit and an extender unit, and (ii) a polyketide producing organism;
- (b) selecting a target concentration range for said precursor compound in said culture medium;
- and
- (c) culturing said polyketide producing organism in said culture medium to produce said polyketide, said culture medium being in contact with a resin that reversibly binds said precursor compound so that the concentration of un-adsorbed precursor compound in said culture medium is maintained within the target concentration range by the release of said precursor compound bound to the resin as said precursor compound is converted to said polyketide.
1 more element
Dependencies are inferred from the claim text.