Cytokine zcytor17 ligand polynucleotides
AssigneeZymoGenetics, Inc.
InventorsCindy A. Sprecher, Joseph L. Kuijper, Maria M. Dasovich, Francis J. Grant, Angela K. Hammond, Jane A. Gross
Life of this patent
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Abstract
The present invention relates to zcytor17lig polynucleotide, polypeptide and anti-zcytor17 antibody molecules. The zcytor17lig is a novel cytokine. The polypeptides may be used within methods for stimulating the immune system, and proliferation and/or development of hematopoietic cells in vitro and in vivo. The present invention also includes methods for producing the protein, uses therefor and antibodies thereto.
Claims35
An isolated polynucleotide molecule comprising the sequence of nucleotides that encode a polypeptide comprising the sequence of amino acid residues that is at least 95% identical to the sequence of amino acid residues of the polypeptide selected from the group consisting of:
- (a) the polypeptide comprising the amino acid sequence from residues 38 (Val) to 152 (Leu) as shown in SEQ ID NO:2;
- (b) the polypeptide comprising the amino acid sequence from residues 27 (Leu) to 164 (Thr) as shown in SEQ ID NO:2;
- (c) the polypeptide comprising the amino acid sequence from residues 24 (Thr) to 164 (Thr) as shown in SEQ ID NO:2;
- and
- (d) the polypeptide comprising the amino acid sequence from residues 1 (Met) to 164 (Thr) as shown in SEQ ID NO:2
- wherein the polypeptide induces proinflammatory cytokine production.
An isolated polynucleotide molecule comprising the sequence of nucleotides that encode a polypeptide comprising at least 14 contiguous amino acid residues of SEQ ID NO:2 wherein the polypeptide is used to produce an antibody.
An expression vector comprising the following operably linked elements:
- (a) a transcription promoter;
- (b) a DNA segment encoding a polypeptide comprising the amino acid sequence from residues 27 to 164 of SEQ ID NO:2;
- and
- (c) a transcription terminator.
An expression vector comprising the following operably linked elements:
- (a) a transcription promoter;
- (b) a DNA segment encoding a polypeptide comprising the sequence of amino acid residues that is at least 95% identical to residues 38 (Val) to 152 (Leu) as shown in SEQ ID NO:2 wherein the polypeptide induces proinflammatory cytokine production;
- and
- (c) a transcription terminator.
An expression vector comprising the following operably linked elements:
- (a) a transcription promoter;
- (b) a DNA segment encoding a polypeptide comprising the amino acid residues 38 (Val) to 152 (Leu) of SEQ ID NO:2;
- and
- (c) a transcription terminator.
An isolated polynucleotide encoding a polypeptide comprising the sequence of amino acid residues from residue 27 to residue 164 as shown in SEQ ID NO:
- 2.
An isolated polynucleotide encoding a polypeptide consisting of the sequence of amino acid residues from residue 27 to residue 164 as shown in SEQ ID NO:
- 2.
An isolated polynucleotide encoding a polypeptide consisting of the sequence of amino acid residues from residue 1 to residue 164 as shown in SEQ ID NO:
- 2.
An isolated polynucleotide encoding a polypeptide comprising the sequence of amino acid residues from residue 38 to residue 152 as shown in SEQ ID NO:
- 2.
An isolated polynucleotide comprising a sequence of nucleotides that encode an antigenic polypeptide comprising a sequence of 30 contiguous amino acid residues of SEQ ID NO:
- 2, wherein the antigenic polypeptide comprises the amino acid sequence selected from the group consisting of:
- (a) amino acid residues 54 to 59 of SEQ ID NO:
- 2;
- (b) amino acid residues 129 to 134 of SEQ ID NO:
- 2;
- (c) amino acid residues 53 to 58 of SEQ ID NO:
- 2;
- (d) amino acid residues 35 to 40 of SEQ ID NO:
- 2;
- (e) amino acid residues 33 to 38 of SEQ ID NO:
- 2;
- (f) amino acid residues 114 to 119 of SEQ ID NO:
- 2;
- (g) amino acid residues 101 to 105 of SEQ ID NO:
- 2;
- (h) amino acid residues 126 to 131 of SEQ ID NO:
- 2;
- (i) amino acid residues 113 to 118 of SEQ ID NO:
- 2;
- and
- (j) amino acid residues 158 to 162 of SEQ ID NO:2
- and wherein the polypeptide induces proinflammatory cytokine production.
Dependencies are inferred from the claim text.