Methods of using functional 30S subunits
AssigneeIowa State University Research Foundation, Inc.
InventorsGloria M. Culver, Jennifer A. Maki
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Abstract
A method of preparing a peptide in vitro in a defined translation mixture is provided. The method includes providing a defined translation reaction mixture comprising isolated and/or purified 30S subunits comprising small subunit ribosomal proteins and 16S rRNA, 50S subunits comprising large subunit ribosomal proteins, 5S rRNA and 23S rRNA, charged tRNAs for at least one amino acid, an isolated RNA template for translation with at least one codon corresponding to the anticodon in the charged tRNAs, and one or more isolated translation factors.
Claims22
A method to prepare a peptide, comprising:
- a) providing a defined translation reaction mixture comprising isolated i) 30S subunits comprising small subunit ribosomal proteins and 16S rRNA, ii) 50S subunits comprising large subunit ribosomal proteins, 5S rRNA and 23S rRNA, iii) charged tRNAs for at least one amino acid, iv) RNA template for translation with at least one codon corresponding to the anticodon in the charged tRNAs, and v) one or more translation factors;
- and
- b) incubating the mixture so as to yield a peptide encoded by the RNA template.
Dependencies are inferred from the claim text.