Method for determination and quantification of radiation or genotoxin exposure

A method of screening test agents which modulate formation of FANCD2-containing foci, said method comprising:

• identifying an individual suspected of having been exposed to a genotoxic agent;
• obtaining a biological sample from said individual;
• contacting said biological sample with test compound;
• and
• detecting the presence of FANCD2 in FANCD2-containing foci relative to a control sample, wherein a difference in the degree of foci formation relative to a control sample is indicative of an agent active in foci formation, wherein said biological sample is a cell expressing a polynucleotide comprising a DNA sequence encoding a protein that binds with FANCD2 upon foci formation, fused in frame with a DNA sequence encoding a second fluorophore.

A method of screening test agents which modulate formation of FANCD2-containing foci, said method comprising:

• identifying an individual suspected of having been exposed to a genotoxic agent;
• obtaining a biological sample from said individual;
• contacting said biological sample with test compound;
• and
• detecting the presence of FANCD2 in FANCD2-containing foci relative to a control sample, wherein a difference in the degree of foci formation relative to a control sample is indicative of an agent active in foci formation, wherein said biological sample is a cell expressing a polynucleotide comprising a DNA sequence encoding the human FACD2 protein of SEQ ID NO:1 fused in frame with a DNA sequence encoding a fluorescent protein and a polynucleotide comprising a DNA sequence encoding a protein that binds with FANCD2 upon foci formation, fused in frame with a DNA sequence encoding a second fluorophore.