Docetaxel immunoassay
AssigneeSaladax Biomedical Inc.
InventorsSalvatore J. Salamone, Jodi Blake Courtney, Gregory Drake Lundell, Mahmoud Ahmed ElSohly, Waseem Gul
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Abstract
Novel conjugates of docetaxel and novel docetaxel immunogens derived from the 7 and 10 positions of docetaxel and monoclonal antibodies generated by these docetaxel linked immunogens are useful in immunoassays for the quantification and monitoring of docetaxel in biological fluids.
Claims18
An immunoassay process for detecting docetaxel in a sample comprising providing a mixture containing a sample, an antibody reactive with docetaxel and not substantially reactive with paclitaxel and 10-O-deacetylbaccatin III, and a compound conjugated with a carrier wherein the compound is selected from those represented by the formula:
- wherein B is
- Y is an organic spacing group;
- X is a functional group capable of linking to a carrier;
- and
- p is an integer from 0 to 1;
- a compound of the formula:
- wherein B, X, Y and p are as above;
- a compound of the formula
- wherein B, X, Y and p are as above,
- and mixtures thereof,
- causing the docetaxel in the sample and said conjugate to bind with said antibody and thereafter measuring the amount of said conjugate in said mixture which is bound or unbound to said antibody whereby the presence of docetaxel in the sample can be determined.
A kit for determining the presence of docetaxel in a patient sample comprising reagents packed in separate containers, one of the reagents being an antibody reactive with docetaxel and not substantially reactive with paclitaxel and 10-O-deacetylbaccatin III, and the other reagent being a compound conjugated with a carrier wherein the compound is selected from those represented by the formula:
- wherein B is
- Y is an organic spacing group;
- X is a functional group capable of linking to a carrier;
- and
- p is an integer from 0 to 1;
- a compound of the formula:
- wherein B, X, Y and p are as above;
- a compound of the formula
- wherein B, X, Y and p are as above;
- and mixtures thereof.
Dependencies are inferred from the claim text.