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US 7,459,275 B2Granted
Sequencing of surface immobilized polymers utilizing microfluorescence detection
AssigneeAffymetrix, Inc.
InventorsWilliam J. Dower, Stephen P. A. Fodor
Life of this patent
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Abstract
Means for simultaneous parallel sequence analysis of a large number of biological polymer macromolecules. Apparatus and methods may use fluorescent labels in repetitive chemistry to determine terminal monomers on solid phase immobilized polymers. Reagents which specifically recognize terminal monomers are used to label polymers at defined positions on a solid substrate.
Claims16
A method for analyzing a target nucleic acid comprising:
- (a) providing a nucleic acid array, said array comprising at least two different target template nucleic acids attached to a support in positionally distinct regions at a density of at least 1000 regions per cm2;
- (b) contacting said nucleic acid array with at least one nucleotide comprising a removable label;
- (c) detecting the label to determine the addition of the nucleotide to an oligonucleotide hybridized to one of the template nucleic acids;
- (d) removing the label;
- and
- (e) repeating steps (b) and (c).
3 more elements
A method for analyzing a target nucleic acid comprising:
- (a) providing a nucleic acid array, said array comprising at least two different target template nucleic acids attached to a support in positionally distinct regions at a density of at least 1000 regions per cm2;
- (b) contacting said nucleic acid array with at least one nucleic acid segment of a defined length;
- and
- (c) determining the addition of the nucleic acid segment to an oligonucleotide hybridized to one of the target template nucleic acids.
1 more element
Dependencies are inferred from the claim text.