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US 7,459,269 B2Granted
Detection of bovine viral diarrhea virus in hair samples
AssigneesCornell Research Foundation, Inc., IDEXX Laboratories, Inc.
InventorsRoy Huchzermeier, Edward Joseph Dubovi
Life of this patent
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Abstract
The present invention relates to a method of detecting whether a target animal is Bovine Viral Diarrhea Virus (BVDV) positive or negative by determining whether a gp48 protein-specific reagent binds to a gp48 Bovine Viral Diarrhea Virus protein or protein fragment, which retains antigenic specificity, from a target animal's hair sample.
Claims22
A method of detecting whether a target animal is Bovine Viral Diarrhea Virus positive or negative comprising:
- a) suspending a hair sample from said target animal in a diluent under conditions effective to solubilize any gp48 Bovine Viral Diarrhea Virus protein or protein fragment thereof;
- b) providing an assay system which comprises:
- 1) a capture antibody that is a Bovine Viral Diarrhea Virus epitope specific antibody, said capture antibody being immobilized on a solid support and capable of recognizing and binding a gp48 Bovine Viral Diarrhea Virus protein or protein fragment thereof which retains antigenic specificity;
- 2) a detector antibody that is an anti-Bovine Viral Diarrhea Virus antibody, capable of recognizing and binding said gp48 Bovine Viral Diarrhea Virus protein or protein fragment thereof which retains antigenic specificity;
- and
- 3) a signal generator for indicating the presence of said detector antibody operatively associated with Bovine Viral Diarrhea Virus antigen;
- c) analyzing said diluent in which the hair sample is suspended with said assay system to generate a change in signal if Bovine Viral Diarrhea Virus antigen which retains its antigenic and epitopic specificity is present in the sample compared to when no such antigen is present;
- and
- d) comparing the signal generated to one or more reference levels to indicate whether the target animal is Bovine Viral Diarrhea Virus positive or negative.
6 more elements
A method of detecting Bovine Viral Diarrhea Virus infection in a bovine comprising:
- suspending a hair sample from the bovine in a diluent under conditions effective to solubilize any gp48 Bovine Viral Diarrhea Virus protein or protein fragment thereof;
- contacting said diluent in which the hair sample is suspended with a reagent which retains the antigenic and epitopic specificity of gp48 Bovine Viral Diarrhea Virus protein;
- analyzing whether the reagent binds to a gp48 Bovine Viral Diarrhea Virus protein or protein fragment thereof from said diluent in which the hair sample is suspended, whereby a change in signal is generated if a Bovine Viral Diarrhea Virus protein or protein fragment, which retains antigenic and epitopic specificity, is present in the sample compared to when no such protein or protein fragment is present;
- and
- comparing the signal generated to one or more reference levels to indicate whether the bovine is infected with Bovine Viral Diarrhea Virus.
2 more elements
Dependencies are inferred from the claim text.