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US 7,459,161 B2Granted
Methods for eliciting an immune response using cytolysin and hemolysin fusion proteins
AssigneeUniversity of Maryland, Baltimore
InventorJames E. Galen
Life of this patent
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Abstract
The disclosure below provides a protein export system for efficiently producing recombinant protein from a host cell. In a preferred embodiment, the protein export system utilizes protein export machinery endogenous to the host bacterium into which the protein export system vector is introduced.
Claims7
A method for eliciting an immune response to a fusion protein in a subject comprising:
- administering to a subject a population of bacteria which produces and exports a fusion protein in an amount sufficient to elicit an immune response in said subject to said fusion protein, wherein said bacteria comprises an expression vector encoding said fusion protein, wherein said fusion protein comprises an export protein linked to a protein of interest in a 5′ to 3′ arrangement, wherein said export protein is Salmonella enterica serovar Typhi (S. Typhi) cytolysin A (ClyA) protein of SEQ ID NO:
- 2 or Escherichia coli hemolysin E (HlyE) protein encoded by the polynucleotide sequence set forth in SEQ ID NO:25, and wherein said bacteria is S. Typhi or E. coli;
- thereby eliciting an immune response to said fusion protein in said subject.
A method for eliciting an immune response to a fusion protein in a subject comprising:
- administering to a subject a population of bacteria which produces and exports a fusion protein in an amount sufficient to elicit an immune response in said subject to said fusion protein, wherein said bacteria comprises an expression vector encoding said fusion protein, wherein said fusion protein comprises an export protein linked to a protein of interest in a 5′ to 3′ arrangement, wherein export protein is Salmonella enterica serovar Typhi (S Typhi) cytolysin A (ClyA) protein of SEQ ID NO:
- 2 with one or more mutations resulting in amino acid substitutions selected from the group consisting of amino acid substitutions at positions 180, 185, 187, and 193, which attenuate the hemolytic activity of the export protein, and wherein said bacteria is S. Typhi E. coli;
- thereby eliciting an immune response to said fusion protein in said subject.
Dependencies are inferred from the claim text.