Methods and reagents for the enhancement of virus transduction in the bladder epithelium
AssigneeCell Genesys, Inc.
InventorsNagarajan Ramesh, David Frey, Bahram Memarzadeh, DeChao Yu
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Abstract
Agents and methods for enhancing recombinant virus transduction in the bladder epithelium are described. A first method involves contacting the luminal surface of the bladder with a composition comprising a transduction enhancing agent and an oncolytic virus. Alternatively, the luminal surface of the bladder can be contacted first with a pretreatment composition comprising a transduction enhancing agent and, subsequently, with a composition comprising an oncolytic virus. Bladder treatment compositions comprising a transduction enhancing agent and an oncolytic virus are also described.
Claims22
A method for treating superficial cancer of the bladder comprising:
- contacting a luminal surface of the bladder with a pretreatment composition comprising a transduction enhancing agent;
- and
- further contacting the luminal surface of the bladder with a composition comprising a replication competent oncolytic adenovirus,
- wherein the transduction enhancing agent is a disaccharide having a lipophilic side chain consisting of 12 carbons;
- wherein said oncolytic adenovirus exhibits preferential expression in bladder epithelium;
- and wherein said cancer is derived from the bladder epithelium.
A composition comprising:
- a transduction enhancing agent;
- and
- a replication competent oncolytic adenovirus;
- wherein said replication competent oncolytic adenovirus is effective to transduce cells of a bladder epithelium and said transduction enhancing agent has the following general formula (I):
- wherein X is a sulfur or oxygen atom, each R2 is independently hydrogen or a moiety represented by:
- and R1 represents an alkyl or alkenyl group of 12 carbons.
A composition comprising:
- a transduction enhancing agent;
- and
- a replication competent oncolytic adenovirus;
- wherein said replication competent oncolytic adenovirus is effective to transduce cells of a bladder epithclium and said transduction enhancing agent has the following formula
- wherein n is 6.
A composition comprising:
- a transduction enhancing agent;
- and
- a replication competent oncolytic adenovirus;
- wherein said replication competent oncolytic adenovirus is effective to transduce cells of a bladder epithelium and said transduction enhancing agent has the following formula
- wherein n is 6.
Dependencies are inferred from the claim text.