Multifunctional magnetic nanoparticle probes for intracellular molecular imaging and monitoring

A composition for facilitating signal transduction in molecular imaging or monitoring, comprising:

• a first magnetic nanoparticle probe composition comprising a detectable moiety comprising a magnetic nanoparticle having a biocompatible coating thereon;
• a first targeting probe attached to the biocompatible coating;
• and a delivery ligand attached to the biocompatible coating;
• and
• a second magnetic nanoparticle probe composition comprising a detectable moiety comprising a magnetic nanoparticle having a biocompatible coating thereon;
• a second targeting probe attached to the biocompatible coating;
• and a delivery ligand attached to the biocompatible coating;
• wherein the first targeting probe binds to a first target and the second targeting probe binds with a second target;
• and wherein an effect on water relaxation from interaction between the first and second magnetic nanoparticles is detected to determine binding of both the first and the second targeting probes to the first and the second target, and wherein the first magnetic nanoparticle probe composition is different than the second magnetic nanoparticle probe composition.

A method for producing a magnetic nanoparticle probe composition for facilitating molecular imaging, comprising:

• combining a magnetic nanoparticle with a biocompatible coating;
• adding an intracellular delivery ligand;
• and
• adding a targeting probe, wherein the targeting probe is selected from the group consisting of a nucleic acid probe, an antibody, an antibody fragment, and an aptamer.

A nanoparticle probe composition for facilitating molecular imaging or monitoring, comprising:

• a detectable moiety comprising a magnetic nanoparticle having a biocompatible coating thereon;
• a targeting probe attached to the biocompatible coating;
• and
• an intracellular delivery ligand attached to the biocompatible coating,
• wherein the intracellular delivery ligand is selected from the group consisting of the HSV VP22 peptide (SEQ ID NO:2), the ANTP peptide (SEQ ID NO:3), the W/R peptide (SEQ ID NO:4), the NLS* peptide (SEQ ID NO:5), the AlkCWK1S peptide (SEQ ID NO:6), the DiCWK18 peptide (SEQ ID NO:7), the transportan peptide (SEQ ID NO:8), the DipaLytic peptide (SEQ ID NO:9), the KI6RGD peptide (SEQ ID NO:1O), the P1 peptide (SEQ ID 10:11), the P2 peptide (SEQ ID NO:12), the P3 peptide (SEQ ID NO:13), the P3a peptide (SEQ ID NO:14), the P9.3 peptide (SEQ ID NO:15), the Plac peptide (SEQ ID NO:16), the Kplae peptide (SEQ ID NQ:17), the cKplae peptide (SEQ ID NO:
• 18), the MGP peptide (SEQ ID NO:
• 19), the HA2 peptide (SEQ ID NO:20), the LARL46 peptide (SEQ ID NO:21), the Hel-l1-7 peptide (SEQ ID NO:22), the KK peptide (SEQ ID NO:23), the KWK peptide (SEQ ID NO:24), the RWR peptide (SEQ ID NO:2 5), and the Loligomer peptide (SEQ ID NO:26).

A method for producing a magnetic nanoparticle probe composition for facilitating molecular imaging, comprising:

• combining a magnetic nanoparticle with a biocompatible coating;
• adding a targeting probe;
• and
• adding an intracellular delivery ligand;
• wherein the intracellular delivery ligand is selected from the group consisting of the HSV VP22 peptide (SEQ ID NO:2), the ANTP peptide (SEQ ID NO:3), the W/R peptide (SEQ ID NO:4), the NLS* peptide (SEQ ID NO:5), the AlkCWKI8 peptide (SEQ ID NO:6), the DiCWK18 peptide (SEQ ID NO:7), the transportan peptide (SEQ ID NO:8), the DipaLytic peptide (SEQ ID NO:9), the K16RGD peptide (SEQ ID NO:10), the P1 peptide (SEQ ID NO:11 ), the P2 peptide (SEQ ID NO:12), the P3 peptide (SEQ ID NO:13), the P3a peptide (SEQ ID NO:14), the P9.3 peptide (SEQ ID NO:15), the Plae peptide (SEQ ID NO:16), the Kplae peptide (SEQ ID NO:17). the cKplae peptide (SEQ ID NO:18), the MGP peptide (SEQ ID NO:19), the HA2 peptide (SEQ ID NO:20), the LARL46 peptide (SEQ ID NO:21), the Hel-11-7 peptide (SEQ ID NO:22), the KK peptide (SEQ ID NO:23), the KWK peptide (SEQ ID NO:24), the Rfl peptide (SEQ ID NO:25), and the Loligomer peptide (SEQ ID N0:26).