Loading patent…
AssigneeCellectricon AB
InventorsFrida Ryttsén, Owe Orwar, Mikael Levin, Eskil Sahlin, Joakim Wigström
Estimating the term…
The invention provides hollow-tip-electrodes for spatially localized delivery of substances to one or more biological targets present in a population comprising target and non-target molecules, macromolecules, and/or cells. The invention also provides electrode plates for receiving one or more of such tips, tip-electrode plates comprising electrode plates comprising one or more electrode tips, and systems comprising tip-electrodes and containers for containing one or more biological targets, e.g., such as molecules, macromolecules, and/or cells. The invention further provides methods for using such systems and components thereof. In one preferred aspect, the systems are used for spatially confined electroporation of cells and cell structures. The invention facilitates high throughput screening of agents (e.g., such as drugs) that act on intracellular targets.
A tip-electrode comprising:
An electrode plate comprising at least one mounting point for receiving a tip electrode, wherein tip electrode comprises a housing defining a lumen for receiving an electrically conductive medium and an electrically conducting surface for coupling to a voltage or current generator.
A tip-electrode plate comprising a substantially planar plate on which at least one non-planar element is fabricated thereon, the non-planar element comprising an electrically conductive medium and wherein the end of the non-planar element distal from the plate comprises an opening for exposing a target to an electric field and for the delivery of an agent from the opening to the target, and wherein inner walls of the non-planar element define a lumen.
A system comprising at least one tip electrode comprising:
The system of 68, wherein the system further comprises a detector, for detecting alteration of electrical properties or optical properties of a target proximity to a tip electrode and/or delivery of a fluid and/or agent to the target.
Dependencies are inferred from the claim text.