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AssigneeElan Pharmaceuticals, Inc.
InventorsJohn P. Anderson, Guriqbal Basi, Minh Tam Doan, Normand Frigon, Varghese John, Michael Power, Sukanto Sinha, Gwen Tatsuno, Jay Tung, Shuwen Wang, Lisa McConlogue
Estimating the term…
Disclosed is an active, isolated β-secretase enzyme in purified and recombinant form. This enzyme is implicated in the production of amyloid plaque components which accumulate in the brains of individuals afflicted with Alzheimer's disease. Recombinant cells that produce this enzyme either alone or in combination with some of its natural substrates (APP and APPsw) are also disclosed. These compositions are useful for use in methods of selecting compounds that modulate β-secretase. Inhibitors of β-secretase are implicated as therapeutics in the treatment of neurodegenerative diseases, such as Alzheimer's disease.
An isolated nucleic acid encoding a beta secretase, wherein the nucleic acid consists of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:
A method of producing a recombinant β-secretase enzyme consisting of SEQ ID NO:
An isolated cell, comprising
An isolated nucleic acid encoding a beta secretase, wherein the nucleic acid consists of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:
An isolated nucleic acid encoding a beta secretase, wherein the nucleic acid consists of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:
An isolated nucleic acid encoding a beta secretase, wherein the nucleic acid consists of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:
An isolated nucleic acid encoding a beta secretase, wherein the nucleic acid consists of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:67 or the full length complementary sequence thereof.
An isolated nucleic acid encoding a beta secretase, wherein the nucleic acid consists of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:
An isolated nucleic acid encoding a beta secretase, the nucleic acid consisting of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:
An isolated nucleic acid encoding a beta secretase, wherein the nucleic acid consists of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:
An isolated nucleic acid encoding a beta secretase, wherein the nucleic acid consists of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:
A method of producing a recombinant β-secretase enzyme consisting of SEQ ID NO:
A method of producing a recombinant β-secretase enzyme consisting of SEQ ID NO:
A method of producing a recombinant β-secretase enzyme consisting of SEQ ID NO:66, comprising culturing a cell transfected with a vector comprising a nucleic acid encoding a beta secretase, the nucleic acid consisting of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:
A method of producing a recombinant β-secretase enzyme consisting of SEQ ID NO:67, comprising culturing a cell transfected with a vector comprising a nucleic acid encoding a beta secretase, the nucleic acid consisting of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:67 under conditions to promote growth of said cell, and subjecting an extract or cultured medium from said cell to an affinity matrix.
A method of producing a recombinant β-secretase enzyme consisting of SEQ ID NO:68, comprising culturing a cell transfected with a vector comprising a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:
A method of producing a recombinant β-secretase enzyme consisting of SEQ ID NO:69, comprising culturing a cell transfected with a vector comprising a nucleic acid encoding a beta secretase, the nucleic acid consisting of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:69 under conditions to promote growth of said cell, and subjecting an extract or cultured medium from said cell to an affinity matrix.
A method of producing a recombinant β-secretase enzyme consisting of SEQ ID NO:
A method of producing a recombinant β-secretase enzyme consisting of SEQ ID NO:
An isolated cell, comprising
An isolated cell, comprising
An isolated cell, comprising
An isolated cell, comprising
An isolated cell, comprising
An isolated cell, comprising
An isolated cell, comprising p1 (i) a nucleic acid molecule encoding a beta secretase, wherein the nucleic acid consists of a nucleotide sequence encoding the beta secretase consisting of SEQ ID NO:70;
An isolated cell, comprising
Dependencies are inferred from the claim text.