Imaging method and apparatus
AssigneeBio-Rad Laboratories, Inc.
InventorCharles W. Ragsdale
Life of this patent
Estimating the term…
Abstract
A method is provided for measuring a response to a stimulus of a plurality of samples spots of a sample using a measuring system having a measurement range to generate an image of the sample in digital space. The method includes, for each sample, while measuring the response, varying the stimulus to include at least one stimulus value where the measured response corresponds to a value in an intermediate portion of the measuring range, and storing a value of the measured response that corresponds to a value in the intermediate portion of the measurement range, and the stimulus value that produced that value of the measured response.
Claims26
A method of measuring a response to a stimulus of a plurality of samples spots of a sample using a measuring system having a measurement range to generate an image of the sample in digital space, the method comprising:
- for each sample,
- while measuring the response, varying the stimulus to include at least one stimulus value where the measured response corresponds to a value in an intermediate portion of the measuring range, and
- storing a value of the measured response that corresponds to a value in the intermediate portion of the measurement range, and the stimulus value that produced that value of the measured response.
A method of acquiring image-response values for an extended sample subjected to a stimulus to generate an image in digital space that includes the image-response values, the method comprising:
- for each of a plurality of spots,
- subjecting the sample to a plurality of stimulus values in a single scan of the spots,
- measuring corresponding response values,
- determining a stimulus value that provides a response value within a desired range, and
- storing the stimulus value, so determined, and the response value provided by that stimulus value;
- providing a normalized data set for the plurality of spots where each spot's normalized value represents a ratio of the stored response value and the corresponding stimulus value.
A method for generating an image of a sample that includes a plurality of spots irradiated with stimulus radiation, such that response radiation from each spot is a response to the stimulus radiation, the method comprising:
- for each spot, in a single scan of the sample:
- varying an intensity value of the stimulus radiation within a discrete range of values,
- storing a radiation value for the response radiation and an attenuation state for that radiation value, wherein the radiation value is within a measurement range of a detector, and each attenuation state is associated an attenuation value, and
- normalizing the radiation values based on the attenuation values to form the image in digital space.
Dependencies are inferred from the claim text.