Fluorescence observation equipment
AssigneeOlympus Corporation
InventorsKen Kawamata, Nobuyoshi Toyohara
Life of this patent
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Abstract
An apparatus for fluorescence observation includes an excitation filter which transmits only exciting light of an specific wavelength among illumination light, and an absorption filter which blocks the exciting light and transmits only fluorescence generated from a specimen when the exciting light is irradiated to the specimen. Here, an interval of a half-value wavelength at a long-wavelength side of the excitation filter and a half-value wavelength at a short-wavelength side of the absorption filter is in a width between 1 nm to 6 nm, and change of the half-value wavelength of the excitation filter and the absorption filter when humidity changes from 10% to 95%, is 0.5 nm or less.
Claims4
An apparatus for fluorescence observation comprising laser light used as exciting light, and an absorption filter which blocks the exciting light and transmits only fluorescence generated from a specimen irradiated with the exciting light,
- wherein the absorption filter includes a multilayer film having 90 or more film layers, and an interval between a wavelength of the laser light and a half-value wavelength of the absorption filter on a short-wavelength side is within a width between 1 nm to 12 nm.
Dependencies are inferred from the claim text.