Reproductive ablation constructs
AssigneeArborgen, LLC
InventorsWilliam H. Rottmann, Kim H. Norris-Caneda, Chunsheng Zhang
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Abstract
The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species. Reproductive-preferred promoters, regulatory elements, and cytotoxic nucleotide sequences are disclosed herein, as are constructs and methods for genetic ablation.
Claims15
A method for obtaining wood pulp, comprising
- (a) introducing into a plant cell of a woody plant a construct comprising (i) a promoter having the sequence of any one of SEQ ID NOs. 4 or 16 operably linked to (ii) a desired nucleic acid that comprises the sequence of SEQ ID NO. 7 or which comprises a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO. 11;
- (b) culturing said transformed plant cell under conditions that promote growth of a plant;
- and
- (c) obtaining wood pulp from said plant.
A construct, comprising (i) the sequence of either SEQ ID NOs. 4 or 16 operably linked to the sequence of SEQ ID NO:
- 7, or (ii) the sequence depicted in SEQ ID NO. 24.
A transgenic plant, comprising a construct that comprises a polynucleotide selected from any one of SEQ ID NO:
- 4 or 16 operably linked to a desired nucleic acid that comprises the sequence of SEQ ID NO. 7 or which encodes the amino acid sequence of SEQ ID NO. 11.
A method for producing a transgenic plant, comprising
- (a) transforming a plant cell with a construct that comprises at least one polynucleotide having the sequence of any one of SEQ ID NOs. 4 or 16 operably linked to a desired nucleic acid, wherein said polynucleotide regulates the activity of said desired nucleic acid that has the sequence of SEQ ID NO. 7 or which encodes the amino acid sequence of SEQ ID NO. 11;
- (b) culturing said transformed plant cell under conditions that promote growth of a plant, wherein said plant is a transgenic plant that exhibits a phenotype that is different from a plant of the same species that does not contain said construct.
A construct comprising a polynucleotide selected from any one of SEQ ID NO. 4 or 16 operably linked to a desired nucleic acid that comprises the sequence of SEQ ID NO. 7 which encodes SEQ ID NO. 11, wherein said polynucleotide regulates the expression of said desired nucleic acid in a plant cell transformed with the construct.
A hybrid progeny plant transformed with a construct comprising the sequence of SEQ ID NO. 24.
A construct, comprising Pinus radiata male cone (PrMC) promoter operably linked to a desired nucleic acid, wherein the PrMC promoter regulates the expression of the desired nucleic acid that encodes an H102E barnase mutant in a plant cell transformed with the construct and wherein the H102E barnase mutant is capable of disrupting reproductive development of at least one of a male reproductive structure in a plant.
A method for producing a transgenic plant, comprising
- (a) transforming a plant cell with a construct that comprises (i) a functional promoter having the sequence of SEQ ID NO. 4 or SEQ ID NO. 16 operably linked to (ii) an H102E mutant barnase-encoding polynucleotide;
- and
- (b) culturing the transformed plant cell under conditions that promote growth of a plant, wherein said plant is a transgenic plant that exhibits a phenotype that is different from a plant of the same species that has not been transformed with the construct.
A method for producing a transgenic plant, comprising
- (a) transforming a plant cell with a construct that comprises (i) a functional PrMC promoter operably linked to (ii) a polynucleotide that comprises the sequence of SEQ ID NO. 7;
- and
- (b) culturing the transformed plant cell under conditions that promote growth of a plant, wherein said plant is a transgenic plant that exhibits a phenotype that is different from a plant of the same species that has not been transformed with the construct.
A method for producing a transgenic plant, comprising
- (a) transforming a plant cell with a construct that comprises the sequence of SEQ ID NO. 24;
- and
- (b) culturing the transformed plant cell under conditions that promote growth of a plant, wherein said plant is a transgenic plant that exhibits a phenotype that is different from a plant of the same species that has not been transformed with the construct.
Dependencies are inferred from the claim text.