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US 7,452,984 B2Granted
Detection of varicella-zoster virus
AssigneeMayo Foundation for Medical Education and Research
InventorsMark J. Espy, Jim Uhl, Thomas F. Smith
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Abstract
The invention provides methods to detect VZV in biological samples using real-time PCR. Primers and probes for the detection of VZV are provided by the invention. Articles of manufacture containing such primers and probes for detecting VZV are further provided by the invention.
Claims3
An article of manufacture, comprising:
- a pair of VZV gene 29 primers, wherein said pair of gene 29 primers comprises a first gene 29 primer and a second gene 29 primer, wherein said first gene 29 primer consists of the sequence 5′-TGT CCT AGA GGA GGT TTT ATC TG-3′ (SEQ ID NO:5) and wherein said second gene 29 primer consists of the sequence 5′-CAT CGT CTG TAA GAC TTA ACC AG-3′ (SEQ ID NO:6);
- a pair of VZV gene 29 probes, wherein said pair of gene 29 probes comprises a first gene 29 probe and a second gene 29 probe, wherein said first gene 29 probe consists of the sequence 5′-GGG AAA TCG AGA AAC CAC CCTATC CGAC-3′ (SEQ ID NO:7) and wherein said second gene 29 probe consists of the sequence 5′-AAG TTC GCG GTA TAA TTG TCA GTG GCG-3′ (SEQ ID NO:8);
- and
- a donor fluorescent moiety and a corresponding acceptor fluorescent moiety.
1 more element
Dependencies are inferred from the claim text.