Molecules of the card-related protein family and uses thereof
AssigneeMillenium Pharmaceuticals, Inc.
InventorJohn Bertin
Life of this patent
Estimating the term…
Abstract
Novel CARD-9, CARD-10, or CARD-11 polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated CARD-9, CARD-10, or CARD-11 proteins, the invention further provides CARD-9, CARD-10, or CARD-11, fusion proteins, antigenic peptides and anti-CARD-9, CARD-10, or CARD-11 antibodies. The invention also provides CARD-9, CARD-10, or CARD-11 nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a CARD-9, CARD-10, or CARD-11 gene has been introduced or disrupted. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided.
Claims9
An isolated polypeptide selected from the group consisting of:
- a) a polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO:
- 11, wherein the fragment comprises the CARD domain of residues 6-112 of SEQ ID NO:
- 11, wherein the fragment binds Bcl-10;
- b) a polypeptide comprising a variant of the amino acid sequence of SEQ ID NO:
- 11, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule consisting of the entire complement of SEQ ID NO:
- 12 under conditions of incubation at 45° C. in 6.0X SSC followed by washing in 0.2X SSC/0.1% SDS at 65° C., wherein the variant binds Bcl-10 and comprises a CARD domain;
- c) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to SEQ ID NO:
- 10, 12, wherein the polypeptide binds Bcl-10 and comprises a CARD domain;
- and
- d) a polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 11, wherein the polypeptide binds Bcl-10 and comprises a CARD domain.
An isolated polypeptide consisting of the amino acid sequence of SEQ ID NO:
- 11.
Dependencies are inferred from the claim text.