Methods for developing conifer somatic embryos
AssigneeWeyerhaeuser Company
InventorsPramod K. Gupta, Diane G. Holmstrom, Bonnie Larson
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Abstract
The present invention provides methods for producing conifer cotyledonary somatic embryos. The methods of the present invention each includes the step of culturing embryogenic conifer tissue in, or on, a development medium for a period of time sufficient to produce conifer cotyledonary somatic embryos from the embryogenic conifer tissue, wherein the volume of development medium is in the range of from about 35 ml to about 50 ml per 0.5 mL of embryogenic conifer tissue.
Claims14
A method for producing conifer cotyledonary somatic embryos, the method comprising the step of culturing embryogenic conifer tissue in, or on, a development medium comprising abscisic acid at a concentration in the range of from about 1 mg/L to about 200 mg/L for a period of time sufficient to produce conifer cotyledonary somatic embryos from the embryogenic conifer tissue, wherein the volume of development medium is in the range of from about 35 ml to about 50 ml per 0.5 mL of embryogenic conifer tissue.
A method for producing Douglas fir cotyledonary somatic embryos, the method comprising the steps of:
- (a) culturing Douglas fir zygotic embryos in, or on, an initiation medium for a period of time sufficient to produce embryonal suspensor masses;
- (b) culturing the embryonal suspensor masses in, or on, a maintenance medium under conditions effective to promote multiplication of the embryonal suspensor masses;
- and
- (c) culturing the multiplied embryonal suspensor masses in, or on, a development medium comprising abscisic acid at a concentration in the range of from about 1 mg/L to about 200 mg/L for a period of time sufficient to produce Douglas fir cotyledonary somatic embryos from the embryonal suspensor masses, wherein the volume of development medium is in the range of from about 35 ml to about 50 ml per 0.5 mL of embryogenic conifer tissue.
A method for producing Loblolly pine cotyledonary somatic embryos, the method comprising the steps of:
- (a) culturing Loblolly pine zygotic embryos in, or on, an initiation medium for a period of time sufficient to produce embryonal suspensor masses;
- (b) culturing the embryonal suspensor masses in, or on, a maintenance medium under conditions effective to promote the multiplication of the embryonal suspensor masses;
- and
- (c) culturing the multiplied embryonal suspensor masses in, or on, a development medium comprising abscisic acid at a concentration in the range of from about 1 mg/L to about 200 mg/L for a period of time sufficient to produce Loblolly pine cotyledonary somatic embryos from the embryonal suspensor masses, wherein the volume of development medium is in the range of from about 35 ml to about 50 ml per 0.5 mL of embryogenic conifer tissue.
Dependencies are inferred from the claim text.