Compositions and methods for sensitizing and inhibiting growth of human tumor cells
AssigneeSt. Jude Children's Research Hospital
InventorsMary K. Danks, Philip M. Potter, Peter J. Houghton
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Abstract
Polynucleotides encoding carboxylesterase enzymes and polypeptides encoded by the polynucleotides which are capable of metabolizing a chemotherapeutic prodrug and inactive metabolites thereof to active drug are provided. Compositions and methods for sensitizing tumor cells to a prodrug chemotherapeutic agent and inhibiting tumor growth with this enzyme are also provided. In addition, screening assay for identification of drugs activated by this enzyme are described.
Claims9
An isolated polynucleotide comprising the cDNA of SEQ ID NO:20, wherein said polynucleotide encodes a carboxylesterase capable of metabolizing a chemotherapeutic prodrug and inactive metabolites thereof to active drug.
An isolated polynucleotide comprising a cDNA of SEQ ID NO:20, wherein said polynucleotide encodes a carboxylesterase of SEQ ID NO:26 and is capable of metabolizing a chemotherapeutic prodrug and inactive metabolites thereof to active drug.
An isolated polynucleotide comprising a fragment of the cDNA of SEQ ID NO:
- 20, wherein said polynucleotide encodes a carboxylesterase that comprises the amino acid sequence 1-559 of SEQ ID NO:21, and is capable of metabolizing a chemotherapeutic prodrug and inactive metabolites thereof to active drug.
Dependencies are inferred from the claim text.