Recombinant bacterial phytases and uses thereof

A feed comprising a recombinant or a synthetic polypeptide having a phytase activity, wherein the recombinant or synthetic polypeptide having a phytase activity is encoded by a nucleic acid having the sequence of:

• (a) SEQ IDNO:
• 1;
• or
• (b) SEQ ID NO:
• 1, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 1 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 1 to 1296;
• wherein T is substituted with U;
• or wherein the recombinant or synthetic polypeptide having a phytase activity has the amino acid sequence of:
• (i) SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 1 to 432.

A method for treating a phytate-containing feed to lower the phytate content in the feed and increasing the amount of inorganic phosphorous in the feed comprising:

• (a) providing a recombinant polypeptide having a phytase activity, wherein said polypeptide:
• (i) is encoded by a nucleic acid having the sequence of SEQ ID NO:
• 1;
• or
• (ii) is encoded by a nucleic acid having the sequence of SEQ ID NO:
• 1, wherein T is substituted with U;
• or
• (iii) is encoded by a nucleic acid having the sequence of SEQ ID NO:
• 1 from nucleotides 1 to 1296;
• or
• (iv) is encoded by a nucleic acid having the sequence of SEQ ID NO:
• 1 from nucleotides 1 to 1296, wherein T is substituted with U;
• or
• (v) has the amino acid sequence of SEQ ID NO:
• 2;
• or
• (vi) has the amino acid sequence of SEQ ID NO:
• 2 from amino acids 1 to 432;
• (b) providing a composition comprising a phytate-containing feed;
• (c) contacting the recombinant polypeptide having a phytase activity of (a) with the composition of (b) under conditions wherein the recombinant polypeptide having a phytase activity catalyzes the hydrolysis of phytate, thereby making a feed lower in phytate content and higher in inorganic phosphorous content.

A method for supplementing the diet of an animal by increasing the amount of inorganic phosphorous in an ingested feed comprising feeding to the animal a composition comprising a recombinant phytase, wherein the recombinant phytase is encoded by a nucleic acid having the sequence of:

• (a) SEQ ID NO:
• 1;
• or
• (b) SEQ ID NO:
• 1, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 1 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 1 to 1296, wherein T is substituted with U;
• or wherein the recombinant phytase has the amino acid sequence of:
• (i) SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 1 to 432.

A food supplement for an animal comprising a composition comprising a recombinant polypeptide having a phytase activity, wherein the recombinant polypeptide having a phytase activity is encoded by a nucleic acid having the sequence of:

• (a) SEQ ID NO:
• 1;
• or
• (b) SEQ ID NO:
• 1, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 1 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 1 to 1296, wherein T is substituted with U;
• or wherein the recombinant polypeptide having a phytase activity has the amino acid sequence of:
• (i) SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 1 to 432.

A drinkable foodstuff comprising a recombinant polypeptide having a phytase activity, wherein the recombinant polypeptide having a phytase activity is encoded by a nucleic acid having the sequence of:

• (a) SEQ ID NO:
• 1;
• or
• (b) SEQ ID NO:
• 1, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 1 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 1 to 1296, wherein T is substituted with U;
• or wherein the recombinant polypeptide having a phytase activity has the amino acid sequence of:
• (i) SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 1 to 432.

A feed comprising a recombinant or a synthetic polypeptide having a phytase activity, wherein the recombinant or synthetic polypeptide having a phytase activity lacks the native signal peptide and comprises a heterologous signal peptide, and wherein said recombinant or a synthetic polypeptide is encoded by a nucleic acid having the sequence of:

• (a) SEQ ID NO:
• 1 except for the nucleotides encoding the amino acid residues 1 to 22 of SEQ ID NO:
• 2;
• or
• (b) SEQ ID NO:
• 1 except for the nucleotides encoding the amino acid residues 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• wherein T is substituted with U;
• or wherein the recombinant or synthetic polypeptide having a phytase activity has the amino acid sequence of:
• (i) SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 23 to 432.

A feed comprising a polypeptide having a phytase activity, wherein said polypeptide lacks the native signal peptide, wherein said polypeptide is made by a method comprising the following steps:

• (a) providing a nucleic acid that:
• (i) comprises the sequence of SEQ ID NO:
• 1 except for the nucleotides that encode amino acid residues 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) comprises the sequence of SEQ ID NO:
• 1 except for the nucleotides that encode amino acid residues 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (iii) comprises the sequence of SEQ ID NO:
• 1 from nucleotides 67 to 1296:
• or
• (iv) comprises the sequence of SEQ ID NO:
• 1 from nucleotides 67 to 1296, wherein T is substituted with U;
• or
• (v) encodes the polypeptide of SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (vi) encodes the polypeptide of SEQ ID NO:
• 2 from amino acids 23 to 432;
• (b) providing a composition comprising a feed;
• (c) expressing the nucleic acid of (a) under conditions which allow expression of the polypeptide having a phytase activity;
• and
• (d) mixing the phytase of (c) with the composition of (b), thereby making a feed comprising a polypeptide having a phytase activity.

A food supplement for an animal comprising a composition comprising a recombinant polypeptide having a phytase activity, wherein the recombinant polypeptide having a phytase activity lacks the native signal peptide, and wherein the recombinant peptide having a phytase activity is encoded by a nucleic acid having the sequence of:

• (a) SEQ ID NO:
• 1 except for the nucleotides that encode amino acid residues 1 to 22 of SEQ ID NO:
• 2;
• or
• (b) SEQ ID NO:
• 1 except for the nucleotides that encode amino acid residues 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 67 to 1296, wherein T is substituted with U;
• or wherein the recombinant polypeptide having a phytase activity has the amino acid Sequence of:
• (i) SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 23 to 432.

A drinkable foodstuff comprising a recombinant polypeptide having a phytase activity, wherein the recombinant polypeptide having phytase activity lacks the native signal peptide, and wherein the recombinant polypeptide having a phytase activity is encoded by a nucleic acid having the sequence of:

• (a) SEQ ID NO:
• 1 except for the nucleotides that encode amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (b) SEQ ID NO:
• 1 except for the nucleotides that encode amino acids 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 67 to 1296, wherein T is substituted with U;
• or wherein the recombinant polypeptide having a phytase activity has the amino acid sequence of:
• (i) SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 23 to 432.

A method for treating a phytate-containing feed to lower the phytate content in the feed and increasing the amount of inorganic phosphorous in the feed comprising the following steps:

• (a) providing a recombinant polypeptide having a phytase activity, wherein said recombinant having a phytase activity lacks the native signal peptide and has a heterologous signal peptide, wherein said recombinant polypeptide having a phytase activity:
• (i) is encoded by a nucleic acid comprising SEQ ID NO:
• 1 except for the nucleotides encoding amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) is encoded by a nucleic acid comprising SEQ ID NO:
• 1 expect for the nucleotides encoding amino acids 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (iii) is encoded by a nucleic acid comprising SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• or
• (iv) is encoded by a nucleic acid comprising SEQ ID NO:
• 1 from nucleotides 67 to 1296, wherein T is substituted with U;
• or
• (v) comprises SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (vi) comprises SEQ ID NO:
• 2 from amino acids 23 to 432;
• (b) providing a composition comprising a phytate-containing feed;
• and
• (c) contacting the recombinant polypeptide having a phytase activity of (a) with the composition of (b) under conditions wherein the polypeptide having a phytase activity catalyzes the hydrolysis of phytate, thereby making a feed lower in phytate content and higher in inorganic phosphorous content.

A feed comprising a recombinant or a synthetic polypeptide having a phytase activity, wherein the recombinant or synthetic polypeptide having a phytase activity lacks the native signal peptide and is encoded by a nucleic acid having the sequence of:

• (a) SEQ ID NO:
• 1 except for the nucleotides encoding the amino acid residues 1 to 22 of SEQ ID NO:
• 2;
• or
• (b) SEQ ID NO:
• 1 except for the nucleotides encoding the amino acid residues 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• wherein T is substituted with U;
• or wherein the recombinant or synthetic polypeptide having a phytase activity has the amino acid sequence of:
• (i) SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 23 to 432.

A feed comprising a polypeptide having a phytase activity, wherein said polypeptide lacks the native signal peptide and comprises a heterologous signal peptide, wherein said polypeptide is made by a method comprising the following steps:

• (a) providing a nucleic acid that:
• (i) comprises the sequence of SEQ ID NO:
• 1 except for the nucleotides that encode amino acid residues 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) comprises the sequence of SEQ ID NO:
• 1 except for the nucleotides that encode amino acid residues 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (iii) comprises the sequence of SEQ ID NO:
• 1 from nucleotides 67 to 1296:
• or
• (iv) comprises the sequence of SEQ ID NO:
• 1 from nucleotides 67 to 1296, wherein T is substituted with U;
• or
• (v) encodes the polypeptide of SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (vi) encodes the polypeptide of SEQ ID NO:
• 2 from amino acids 23 to 432;
• (b) providing a composition comprising a feed;
• (c) expressing the nucleic acid of (a) under conditions which allow expression of the polypeptide having a phytase activity;
• and
• (d) mixing the phytase of (c) with the composition of (b), thereby making a feed comprising a polypeptide having a phytase activity.

A method for treating a phytate-containing feed to lower the phytate content in the feed and increasing the amount of inorganic phosphorous in the feed comprising the following steps:

• (a) providing a recombinant polypeptide having a phytase activity, wherein said recombinant having a phytase activity lacks the native signal peptide, wherein said recombinant polypeptide having a phytase activity:
• (i) is encoded by a nucleic acid comprising SEQ ID NO:
• 1 except for the nucleotides encoding amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) is encoded by a nucleic acid comprising SEQ ID NO:
• 1 expect for the nucleotides encoding amino acids 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (iii) is encoded by a nucleic acid comprising SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• or
• (iv) is encoded by a nucleic acid comprising SEQ ID NO:
• 1 from nucleotides 67 to 1296, wherein T is substituted with U;
• or
• (v) comprises SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (vi) comprises SEQ ID NO:
• 2 from amino acids 23 to 432;
• (b) providing a composition comprising a phytate-containing feed;
• and
• (c) contacting the recombinant polypeptide having a phytase activity of (a) with the composition of (b) under conditions wherein the polypeptide having a phytase activity catalyzes the hydrolysis of phytate, thereby making a feed lower in phytate content and higher in inorganic phosphorous content.

A method for supplementing the diet of an animal by increasing the amount of inorganic phosphorous in an ingested feed comprising feeding to the animal a composition comprising a recombinant phytase, wherein the recombinant phytase lacks the native signal peptide, and wherein said recombinant phytase is encoded by a nucleic acid having the sequence of:

• (a) SEQ ID NO:
• 1 except for the nucleotides encoding the amino acid residues 1 to 22 of SEQ ID NO:
• 2;
• or
• (b) SEQ ID NO:
• 1 except for the nucleotides encoding the amino acid residues 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 67 to 1296, wherein T is substituted with U;
• or wherein the recombinant phytase has the amino acid sequence of:
• (i) SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 23 to 432.

A method for supplementing the diet of an animal by increasing the amount of inorganic phosphorous in an ingested feed comprising feeding to the animal a composition comprising a recombinant phytase, wherein the recombinant phytase lacks the native signal peptide and comprises a heterologous signal peptide, and wherein said recombinant phytase is encoded by a nucleic acid having the sequence of:

• (a) SEQ ID NO:
• 1 except for the nucleotides encoding the amino acid residues 1 to 22 of SEQ ID NO:
• 2;
• or
• (b) SEQ ID NO:
• 1 except for the nucleotides encoding the amino acid residues 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 67 to 1296, wherein T is substituted with U;
• or wherein the recombinant phytase has the amino acid sequence of:
• (i) SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 23 to 432.

A food supplement for an animal comprising a composition comprising a recombinant polypeptide having a phytase activity, wherein the recombinant polypeptide having a phytase activity lacks the native signal peptide and comprises a heterologous signal peptide, and wherein the recombinant peptide having a phytase activity is encoded by a nucleic acid having the sequence of:

• (a) SEQ ID NO:
• 1 except for the nucleotides that encode amino acid residues 1 to 22 of SEQ ID NO:
• 2;
• or
• (b) SEQ ID NO:
• 1 except for the nucleotides that encode amino acid residues 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 67 to 1296, wherein T is substituted with U;
• or wherein the recombinant polypeptide having a phytase activity has the amino acid sequence of:
• (i) SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 23 to 432.

A drinkable foodstuff comprising a recombinant polypeptide having a phytase activity, wherein the recombinant polypeptide having phytase activity lacks the native signal peptide and comprises a heterologous signal peptide, and wherein the recombinant polypeptide having a phytase activity is encoded by a nucleic acid having the sequence of:

• (a) SEQ ID NO:
• 1 except for the nucleotides that encode amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (b) SEQ ID NO:
• 1 except for the nucleotides that encode amino acids 1 to 22 of SEQ ID NO:
• 2, wherein T is substituted with U;
• or
• (c) SEQ ID NO:
• 1 from nucleotides 67 to 1296;
• or
• (d) SEQ ID NO:
• 1 from nucleotides 67 to 1296, wherein T is substituted with U;
• or wherein the recombinant polypeptide having a phytase activity has the amino acid sequence of:
• (i) SEQ ID NO:
• 2 except for amino acids 1 to 22 of SEQ ID NO:
• 2;
• or
• (ii) SEQ ID NO:
• 2 from amino acids 23 to 432.