Dehydrogenase and a gene encoding the same
AssigneeMitsubishi Chemical Corporation
InventorsNobuyoshi Esaki, Hisaaki Mihara, Mari Hara, Makoto Ueda
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Abstract
The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.
Claims8
An isolated dehydrogenase isolated from Pseudomonas putida having the following physicochemical properties:
- (1) effect:
- to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme;
- (2) substrate specificity:
- to show activity to alkylamine or dialkylamine but not to ammonium;
- (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10;
- (4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5;
- and
- (5) a molecular weight of approximately 36 kiloDaltons in SDS-polyacrylamide electrophoresis.
An isolated polypeptide as in (1) or (2) below
- (1) a polypeptide comprising SEQ ID NO:
- 1;
- or
- (2) a polypeptide having an amino acid sequence wherein 1 to 20 amino acids are deleted, substituted and/or added in the amino acid sequence comprising SEQ ID No. 1 and having N-methyl-L-amino acid dehydrogenase activity.
Dependencies are inferred from the claim text.