Amplification of DNA in a hairpin structure, and applications
AssigneeDana-Farber Cancer Institute, Inc.
InventorG. Mike Makrigiorgos
Life of this patent
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Abstract
The present invention is directed to a hairpin nucleic acid structure and its use. In a preferred embodiment, the hairpin nucleic acid structure can be used in a method of amplification of a template nucleic acid sequence that substantially reduces polymerase-induced errors.
Claims10
A method of amplifying a hairpin structure comprising converting a double stranded nucleic acid into the hairpin structure, wherein the double stranded nucleic acid contains at least one sequence of interest, and is referred to as a template nucleic acid, and wherein the template nucleic acid has an upper strand with a 5′ and a 3′ end and a lower strand with a 5′ and a 3′ end, the method comprising:
- (a) ligating a first single stranded nucleic acid to the 5′ end of the upper strand of the template nucleic acid,
- (b) ligating a second single stranded nucleic acid, which is non-complementary to the first single stranded nucleic acid to the 3′ end of the lower strand of the nucleic acid,
- (c) ligating a cap of single stranded nucleic acid to both the 5′ end of the lower strand and the 3′ end of the upper strand of the template nucleic acid, such that the 3′ end of the upper strand and the 5′ end of the lower strand are contiguous, thereby creating the hairpin structure;
- and further comprising performing polymerase chain reaction with a first primer that binds to at least a portion of the upper single stranded non-complementary region at the 5′ end of the upper strand, and a second primer that binds to at least a portion of the lower single stranded non-complementary region at the 3′ end of the lower strand.
A method of amplifying a nucleic acid sequence of interest that generates a PCR-amplified product which is substantially free of polymerase-induced errors, comprising:
- (a) providing a sequence of interest comprising a double stranded nucleic acid, referred to as a template nucleic acid, wherein the template nucleic acid has an upper strand with a 5′ and a 3′ end and a lower strand with a 5′ and a 3′ end,
- (b) converting the nucleic acid sequence of interest into a first hairpin DNA structure by ligating a first single stranded nucleic acid to the 5′ end of the upper strand of the template nucleic acid, ligating a second single stranded nucleic acid, which is non-complementary to the first single stranded nucleic acid to the 3′ end of the lower strand of the nucleic acid, and ligating a cap of a single stranded nucleic acid to both the 5′ end of the lower strand and the 3′ end of the upper strand of the template nucleic acid, such that the 3′ end of the upper strand and the 5′ end of the lower strand are contiguous, thereby creating the first hairpin structure;
- (c) amplifying the first hairpin DNA structure using PCR with a first primer that binds to at least a portion of the first single stranded nucleic acid, and a second primer that binds to at least a portion of the second single stranded nucleic acid to produce a plurality of linear double stranded PCR products, wherein the double stranded PCR product comprises an amplified sequence of interest and its complementary sequence flanked 5′ and 3′ by the first and the second single-stranded nucleic acid sequences;
- (d) converting the linear double stranded PCR products into a plurality of second hairpin structures by a method which induces denaturation of the linear double stranded PCR products into single stranded PCR products, followed by sudden renaturation, wherein the amplified sequence of interest and its complement within each single strand hybridize during renaturation, thereby forming a hairpin structure;
- (e) identifying from the second hairpin structures mismatch containing hairpin structures that comprise gaps in binding between the sequence of interest and its complementary sequence in the double-stranded region of the second hairpin structure wherein the gaps are a result of polymerase-generated nucleotide changes, insertions, or deletions, and
- (f) removing such mismatch containing hairpin structure, and collecting the DNA that contains no mismatches.
Dependencies are inferred from the claim text.