Methods for increasing polypeptide production
AssigneeImmunex Corporation
InventorsKirk P Van Ness, Michael T Trentalange, Bradley D Dell, Jeffrey T McGrew
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Abstract
The invention provides methods of increasing the production of polypeptides, optionally recombinant polypeptides, from mammalian cells using xanthine derivatives or hybrid polar compounds and cultures containing the same. Combinations of inducers including a hybrid polar compound and/or a xanthine derivative and/or an alkanoic acid can also be used, optionally at temperatures less than 37° C.
Claims27
A method for producing a recombinant polypeptide comprising culturing a mammalian cell line that can produce the polypeptide, wherein the mammalian cell line is cultured at a temperature less than 37° C. in a medium comprising a hybrid polar compound.
A method for producing a polypeptide comprising
- culturing a CHO cell line that can produce the polypeptide, wherein the CHO cell line is cultured at a temperature of less than 37° C. in a medium comprising a hybrid polar compound.
A method for producing a polypeptide comprising:
- culturing a mammalian cell line in a growth phase followed by a production phase, wherein the production phase occurs at a temperature of less than 37° C.;
- and
- adding to the culture medium during the production phase a xanthine derivative;
- wherein the mammalian cell line is selected from the group consisting of a mammalian cell line that has been genetically engineered to produce the polypeptide and a hybridoma cell line that produces an antibody.
A method for producing a recombinant polypeptide comprising:
- culturing a CHO cell line that has been genetically engineered to produce the recombinant polypeptide;
- and
- adding to the culture medium at least one xanthine derivative selected from the group consisting of theobromine and caffeine.
Dependencies are inferred from the claim text.