Method of detecting mild impaired glucose tolerance or insulin secretory defect
AssigneeAsahi Kasei Pharma Corporation
InventorsMasaru Yamakoshi, Takuji Kouzuma
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Abstract
It is intended to provide a noninvasive method of conveniently detecting mild impaired glucose tolerance and/or insulin hyposecretion at the early stage with the use of an enzyme. Namely, mild impaired glucose tolerance and/or hyposecretion at the early stage are detected by quantifying myoinositol secreted into the urine before loading glucose and after loading glucose for a definite period of time with the use of a reagent and comparing the increase (or the increase ratio) in the myoinositol content thus measured with a characteristic level which has been preliminarily determined in normal subjects.
Claims12
A method of detecting mild impaired glucose tolerance or an insulin secretory defect in a subject, wherein the method comprises:
- providing urine samples from said subject, wherein the samples are obtained before and after glucose load, or before and after a meal;
- quantitatively determining the myo-inositol level in the samples;
- and
- determining that the subject has mild impaired glucose tolerance or that the subject has an insulin secretory defect based on the concentration of myo-inositol in the samples,
- wherein the increment of the concentration of myo-inositol at a characteristic value or higher than a characteristic value of 0 to 20 μg myo-inositol per mg creatinine when measured as an increasing amount of myo-inositol excreted in the urine in a period from 0.5 to 6 hours after 75 g glucose load indicates the subject has mild impaired glucose tolerance or the subject has an insulin secretory defect.
Dependencies are inferred from the claim text.