Branched peptide amphiphiles, related epitope compounds and self assembled structures thereof
AssigneeNorthwestern University
InventorsSamuel I. Stupp, Mustafa O. Guler
Life of this patent
Estimating the term…
Abstract
Branched peptide amphiphilic compounds incorporating one or residues providing a pendant amino group for coupling one or more epitope sequences thereto, such compounds and related compositions for enhanced epitope presentation.
Claims21
A non-linear peptide amphiphile compound comprising a peptide component having the formula KXXXAAAK (SEQ ID NO:
- 10), wherein X is G or L, which peptide component further has at least one pendant lysine residue coupled to a component non-linear with said peptide component, wherein the component non-linear with said peptide component is selected from IKVAV (SEQ ID NO:
- 3), YIGSR (SEQ ID NO:
- 4), RGDS (SEQ ID NO:
- 1), and PHSRN (SEQ ID NO:
- 2), wherein said peptide component is coupled to a hydrophobic component, and wherein the hydrophobic component is an alkyl moiety ranging from C6-C22.
A system comprising a non-linear peptide amphiphile compound comprising a peptide component, a hydrophobic component coupled to said peptide component at its N-terminus, said peptide component comprising a peptide of the formula KXXXAAAK (SEQ ID NO:
- 11), wherein X is L or K, further comprising at least one pendant lysine residue which is coupled to a sequence selected from IKVAV (SEQ ID NO:
- 3), YIGSR (SEQ ID NO:
- 4), RGDS (SEQ ID NO:
- 1), and PHSRN (SEQ ID NO:
- 2), wherein the hydrophobic component is an alkyl moiety ranging from C6-C22, and wherein said compound is in a fluid medium.
A method of using a pendant lysine residue of a peptide amphiphile to present a biologically active epitope sequence, said method comprising:
- providing a plurality of peptide amphiphile compounds, each of said compounds comprising a peptide component having the formula KXXXAAAK (SEQ ID NO:10), wherein X is G or L, and a hydrophobic component selected from an alkyl moiety ranging from C6-C22, at least one of said compounds comprising at least one pendant lysine residue;
- and coupling a biologically active epitope sequence selected from IKVAV (SEQ ID NO:3), YIGSR (SEQ ID NO:4), RGDS (SEQ ID NO:1), PHSRN (SEQ ID NO:2) to said pendant lysine residue, wherein said epitope sequence is non-linear to said peptide component.
Dependencies are inferred from the claim text.