Synthesis of DNA
AssigneeLawrence Livermore National Security, LLC
InventorRaymond P. Mariella, Jr.
Life of this patent
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Abstract
A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.
Claims21
A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence using a computer program, a voltage controlled surface, and a solution, consisting of the steps of:
- using a computer program to analyze the desired double-stranded DNA and determine an initial sequence segment and individual preselected sequence segments that when assembled will complete the desired double-stranded DNA,
- providing said initial sequence segment and
- providing said individual preselected segment sequences of DNA that when assembled will complete the desired double-stranded DNA, and
- assembling said individual preselected segment sequences of DNA to produce the desired double-stranded DNA by multiple substeps of assembling said individual preselected segment sequences of DNA that complete the desired double-stranded DNA to produce the desired double-stranded DNA wherein said multiple substeps include
- tethering said initial individual preselected segment sequences of DNA to the voltage controlled surface,
- applying a voltage to said initial individual preselected segment sequences of DNA,
- placing said individual preselected segment sequences of DNA in the solution,
- drawing said individual preselected segment sequences of DNA in solution to said initial individual preselected segment sequences of DNA, and
- ligating said individual preselected segment sequences of DNA to produce the DNA of a desired length and of a predetermined sequence.
A method of synthesizing DNA of a desired length and of a predetermined sequence using a computer program, a voltage controlled surface, and a solution, consisting of the steps of:
- using the computer program to analyze the desired double-stranded DNA and preselecting an initial segment of the DNA of desired length and of a predetermined sequence,
- using the computer program to analyze the desired double-stranded DNA and preselecting a multiplicity of individual DNA sequence segments that will comprise the DNA of a desired length and of a predetermined sequence,
- assembling said initial segment of DNA and said individual preselected segment sequences of DNA to produce the desired double-stranded DNA by multiple substeps that complete the desired double-stranded DNA, said multiple substeps including
- providing said initial segment of DNA,
- providing said individual preselected segment sequences of DNA that when assembled will complete the desired double-stranded DNA,
- tethering said initial segment of DNA to the voltage controlled surface,
- applying a voltage to said initial segment of the DNA of desired length and of a predetermined sequence by applying a voltage to said voltage controlled surface,
- placing said individual preselected segment sequences of DNA in the solution,
- drawing said individual preselected segment sequences of DNA in solution to said initial individual preselected segment sequences of DNA, and
- ligating said individual preselected DNA sequence segments to produce the DNA of a desired length and of a predetermined sequence.
A method of synthesizing desired double-stranded long DNA of a predetermined length and of a predetermined sequence using a computer program, a voltage controlled surface, and a solution, consisting of the steps of:
- using the computer program to analyze the desired double-stranded DNA and for preselecting an initial segment of DNA of a preselected length and of a predetermined sequence and for preselecting individual segment sequences of DNA to produce the desired double-stranded DNA by multiple substeps of assembling said individual preselected segment sequences,
- assembling said initial segment of DNA and said individual preselected segment sequences of DNA to produce the desired double-stranded DNA by multiple substeps that complete the desired double-stranded DNA, said multiple substeps including
- providing said initial segment of DNA,
- providing said individual preselected segment sequences of DNA that when assembled will complete the desired double-stranded DNA,
- tethering said initial segment of DNA to the voltage controlled surface,
- placing said individual preselected segment sequences of DNA in the solution,
- applying a voltage to said initial segment of DNA by applying a voltage to said voltage controlled surface,
- drawing said individual preselected segment sequences of DNA in solution to said initial individual preselected segment sequences of DNA,
- ligating said individual preselected sequence segments to produce the DNA of a desired length and of a predetermined sequence.
Dependencies are inferred from the claim text.