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US 7,452,665 B2Granted
DNA polymerases with enhanced length of primer extension
AssigneeTakara Bio Inc.
InventorWayne M. Barnes
Life of this patent
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Abstract
A formulation and kit of thermostable or other DNA polymerases comprising at least one thermostable or other DNA polymerase which lacks 3′-exonuclease activity, and at least one thermostable DNA polymerase exhibiting 3′-exonuclease activity. Also provided is an improved method for enzymatic extension of DNA strands, especially while, but not limited to, amplifying nucleic acid sequences by polymerase chain reaction wherein the above formulation is made and used to catalyze primer extension.
Claims5
A kit for the synthesis of a polynucleotide, said kit comprising A mixture comprising:
- (a) a first DNA polymerase, wherein said first polymerase possesses 3′-5′ exonuclease activity selected from the group consisting of Archaebacterial DNA polymerases, and
- (b) a second DNA polymerase, wherein said second polymerase lacks 3′-5′ exonuclease activity selected from the group consisting of thermostable DNA polymerases lacking 3′-5′ exonuclease activity;
- wherein the ratio of DNA polymerase activity of the first DNA polymerase to the DNA polymerase activity of the second DNA polymerase is from about 1:100 up to about 1:600.
Dependencies are inferred from the claim text.