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US 7,452,663 B2Granted
Kits for detection of ATP
AssigneePromega Corporation
InventorsKeith Wood, Rita Hannah, Richard A. Moravec
Life of this patent
Estimating the term…
Abstract
Methods and kits for detecting the presence of ATP, for measuring ATP concentrations, and for detecting viable cells using a composition comprising an ATP-dependent enzyme and one or more ATPase inhibitors.
Claims44
A kit for detecting ATP comprising:
- a luciferase component comprising a chemostable luciferase including the amino acid sequence selected from the group consisting of:
- SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4;
- and
- an ATPase inhibitor component comprising one or more ATPase inhibitors.
1 more element
A kit for detecting ATP comprising:
- a luciferase component comprising the amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1, 2, 3, and 4;
- and an ATPase inhibitor component comprising one or more ATPase inhibitors, where the combination of the luciferase component and the ATPase inhibitor component forms a reagent composition for detecting ATP in a sample;
- where the reagent composition maintains at least 30% activity, as measured by luminescence after the reagent composition is combined with the sample, for at least one hour after the reagent composition is combined with a sample containing ATP, as compared to the reagent composition's activity just after the luciferase is combined with the one or more ATPase inhibitors, and where the one or more ATPase inhibitors present in the reagent composition are collectively able to reduce ATPase activity endogenous to the sample by at least 25% relative to the sample's ATPase activity in the absence of the one or more ATPase inhibitors.
1 more element
Dependencies are inferred from the claim text.