Immobilization of bead-displayed ligands on substrate surfaces
OwnerSukanta Banerjee, Hui Huanginventors
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Abstract
Disclosed are two different approaches for immobilization of beads on a substrate, one of which involves forming a bead-nanoparticle composite by cross-linking of the bead mixture with nanoparticles. The other method involves surface modification of the substrate, using multi-layered polyelectrolytes. With either method, the beads are immobilized on the substrate in a manner suitable for viewing, as when the beads are used in assays and need to be analyzed following the assay. Different designs of depressions are also disclosed, some of which are suitable for holding beads in place without any deposition of nanoparticles or polyelectrolytes.
Claims16
A method of affixing an array of microparticles in a series of recesses in a substrate surface, comprising:
- placing microparticles in said series of recesses in a manner such that one microparticle resides in any one recess;
- placing a nanoparticle suspension on the substrate surface over said series of recesses having microparticles therein;
- incubating the nanoparticle suspension with the microparticle containing substrate whereby numerous nanoparticles get deposited and wedged between the microparticle and the walls of the recess in which it resides.
Dependencies are inferred from the claim text.