US 7,452,507 B2Granted

Portable apparatus for separating sample and detecting target analytes

AssigneeSandia Corporation

InventorsRonald F. Renzi, Karl Wally, Robert W. Crocker, James F. Stamps, Stewart K. Griffiths, Julia A. Fruetel, Brent A. Horn, Isaac R. Shokair, Daniel D. Yee, Victoria A. VanderNoot, Boyd J. Wiedenman, Jason A. A. West, Scott M. Ferko

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Abstract

Portable devices and methods for determining the presence of a target analyte using a portable device are provided. The portable device is preferably hand-held. A sample is injected to the portable device. A microfluidic separation is performed within the portable device and at least one separated component detected by a detection module within the portable device, in embodiments of the invention. A target analyte is identified, based on the separated component, and the presence of the target analyte is indicated on an output interface of the portable device, in accordance with embodiments of the invention.

Claims21

Dependent claims

A modular apparatus for detecting a target analyte, the apparatus comprising:

a reservoir module comprising:
a fluid manifold base defining a plurality of bays and a plurality of outlets, each outlet extending between one of the plurality of bays and a lower surface of the fluid manifold base;
and
a plurality of reservoirs coupled to the fluid manifold base positioned within one of the plurality of bays, wherein at least one reservoir comprises a reservoir seal and wherein at least one needle penetrates said reservoir seal;
a microfluidic chip comprising:
a planar upper surface and a planar lower surface, the planar upper surface abutting the lower surface of the fluid manifold base;
a plurality of inlets piercing the upper surface, the inlets positioned in locations corresponding to the plurality of outlets of the fluid manifold base and each in fluid communication with one of the inlets,
wherein said at least one needle is in fluidic communication with at least one inlet;
and
a separation channel embedded within the microfluidic chip between the upper and lower surfaces thereof the separation channel in fluid communication with at least one of said inlets;
a sample introduction port embedded within the microfluidic chip between the upper and lower surfaces thereof, the sample introduction port in fluid communication with said reservoir module and at least one of the plurality of inlets;
a seal positioned between the fluid manifold base and the microfluidic chip, the seal defining at least one area of fluidic communication between one of said plurality of outlets and one of said inlets;
a detection module positioned to interrogate, at least a portion of the separation channel;
and
an output interface in communication with said detection module to indicate detection of the target analyte;
and
a power module for providing controlled current or voltage.

14 more elements

Dependencies are inferred from the claim text.

Facts

Application: 10/633,871Filed 4 Aug 2003 · us provisional application
Priority: 2 Aug 2002
Published: US 2004/0126279 A11 Jul 2004
Granted: 18 Nov 2008
Term ends: Not estimatedThe source records don’t support an estimate.

Classification12 codes

G01N 21/645
Fluorescence; Phosphorescence: specially adapted constructive features of fluorimeters
B01L 3/502715
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
B01L 9/527
Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips: for microfluidic devices, e.g. used for lab-on-a-chip
B01L 3/5027
With fluid transport, e.g. in multi-compartment structures: by integrated microfluidic structures, i.e. dimensions of channels and chambers are…