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US 7,452,453 B2Granted
System for pH-neutral stable electrophoresis gel
AssigneeInvitrogen Corporation
InventorsTimothy V Updyke, Sheldon C Engelhorn
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Abstract
A gel and buffer system for gel electrophoresis wherein separation occurs at neutral pH.
Claims66
A discontinuous buffer gel electrophoresis system comprising:
- an electrophoresis slab gel comprising a separating gel having between 8 and 25 (wt/vol) % acrylamide, and a gel buffer having a pH between 6 and 8, wherein the gel buffer comprises bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane;
- an anode buffer;
- and
- a cathode buffer solution comprising 3-(N-morpholino)propanesulfonic acid (MOPS).
1 more element
A method for performing gel electrophoresis, comprising performing electrophoresis to separate a protein using a discontinuous buffer gel electrophoresis system comprising:
- an electrophoresis slab gel comprising a separating gel having between 8 and 25 (wt/vol) % acrylamide, and a gel buffer having a pH between 6 and 8, wherein the gel buffer comprises bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane;
- an anode buffer;
- and
- a cathode buffer solution comprising 3-(N-morpholino)propanesulfonic acid (MOPS).
1 more element
A discontinuous buffer gel electrophoresis system comprising:
- an electrophoresis slab gel comprising a separating gel having between 8 and 25 (wt/vol) % acrylamide, and a gel buffer having a pH between 6 and 8, wherein the gel buffer is bis(2-hydroxyethyl)iminotris (hydroxymethyl)methane;
- an anode buffer;
- and
- a cathode buffer solution comprising 3-(N-morpholino)propanesulfonic acid (MOPS).
1 more element
Dependencies are inferred from the claim text.