Truncated forms of pullulanase
AssigneeGenencor International, Inc.
InventorsBrian S. Miller, Jayarama K. Shetty
Life of this patent
Estimating the term…
Abstract
The present invention relates to modified pullulanases useful in the starch industry. The present invention provides methods for producing the modified pullulanase, enzymatic compositions comprising the modified pullulanase, and methods for the saccharification of starch comprising the use of the enzymatic compositions.
Claims24
An isolated or purified truncated pullulanase comprising a deletion of 100 amino acids from the amino terminus of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2, wherein said truncated pullulanase is capable of catalyzing the hydrolysis of an alpha-1,6-glucosidic bond.
An isolated or purified truncated pullulanase comprising a deletion of 200 amino acids from the amino terminus of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2, wherein said truncated pullulanase is capable of catalyzing the hydrolysis of an alpha-1,6-glucosidic bond.
An isolated or purified truncated pullulanase comprising a deletion of 300 amino acids from the amino terminus of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2, wherein said truncated pullulanase is capable of catalyzing the hydrolysis of an alpha-1,6-glucosidic bond.
An isolated or purified truncated pullulanase comprising a deletion of 98 amino acids from the amino terminus of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2, wherein said truncated pullulanase is capable of catalyzing the hydrolysis of an alpha-1,6-glucosidic bond.
An isolated or purified truncated pullulanase comprising a deletion of 102 amino acids from the amino terminus of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2, wherein said truncated pullulanase is capable of catalyzing the hydrolysis of an alpha-1,6-glucosidic bond.
An isolated or purified truncated pullulanase produced by a method comprising the steps of:
- a) obtaining a recombinant host cell comprising a nucleic acid encoding a truncated variant of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2,
- b) culturing said host cell under conditions suitable for the production of the truncated pullulanase, and
- c) recovering the truncated pullulanase, wherein the truncated pullulanase comprises a deletion of 98 or 102 amino acids from the amino terminus of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2 and is capable of catalyzing the hydrolysis of an alpha-1,6-glucosidic bond.
An enzymatic composition comprising an isolated or purified truncated pullulanase selected from
- a) a truncated pullulanase comprising a deletion of 100 amino acids from the amino terminus of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2,
- b) a truncated pullulanase comprising a deletion of 200 amino acids from the amino terminus of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2,
- c) a truncated pullulanase comprising a deletion of 300 amino acids from the amino terminus of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2,
- d) a truncated pullulanase comprising a deletion of 98 amino acids from the amino terminus of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2, and
- e) a truncated pullulanase comprising a deletion of 102 amino acids from the amino terminus of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2,
- wherein said truncated pullulanase is capable of catalyzing the hydrolysis of an alpha-1,6-glucosidic bond.
An isolated or purified truncated pullulanase produced by culturing a recombinant host cell in a culture medium under conditions suitable for the production of said truncated pullulanase, wherein said host cell comprises a nucleic acid encoding a truncated variant of the mature form of the Bacillus deramificans pullulanase of SEQ ID NO:2, wherein said truncated pullulanase is capable of catalyzing the hydrolysis of an alpha-1,6-glucosidic bond, and wherein said truncated pullulanase comprises a deletion of 98 or 102 amino acids from the N-terminus of the mature pullulanase.
Dependencies are inferred from the claim text.