Device including inductively heatable fluid retainment region, and method

A fluid processing device to perform real-time PCR on a sample, the device comprising:

• a substrate comprising a top surface, wherein the substrate is adapted to heating and cooling the sample;
• a plurality of fluid retainment regions disposed in or on the substrate for containing the sample, wherein the sample comprises PCR master mix and magnetic particulates;
• one or more conductors in heat-transfer communication with at least one of the plurality of fluid retainment regions, wherein each of the one or more conductors comprises a material adapted to be inductively heated by a magnetically-induced electric current, wherein at least one of the conductors are contained in the magnetic particulates;
• and
• a cover layer disposed on the top surface closing at least one of the plurality of fluid retainment regions, wherein the cover layer is optically clear for detection of real-time PCR.