System for collecting information on biological particles

A system for collecting biological information on a dyed biological particle by irradiating light onto liquid containing the dyed biological particles and detecting information light therefrom, the system comprising:

• a path-defining structure of transparent material defining a flow path through which the liquid containing the dyed biological particles runs;
• an irradiation apparatus for irradiating light onto the particles passing through the flow path;
• a first detecting apparatus for detecting first information light obtained from the irradiated particle;
• and
• a second detecting apparatus for detecting second information light obtained from the irradiated particle;
• wherein at least one of said first and second detecting apparatuses includes one or more fiber optics, of which one ends are arranged adjacent the flow oath so as to collect the information light;
• wherein the flow path defined by said path-defining structure has a rectangular cross section defined by a pair of first walls and a pair of second walls perpendicular to the first walls;
• wherein said irradiation apparatus emits light to one of the first walls;
• wherein said first detecting apparatus detects light transmitting through another one of the first walls;
• wherein said second detecting apparatus detects light transmitting through one of the second walls;
• wherein said path-defining structure includes a partition plate between one ends of the fiber optics and one of the walls opposing to one ends of the fiber optics;
• and
• wherein one ends of the fiber optics opposes to the partition plate.

A system for collecting biological information on a dyed biological particle by irradiating light onto liquid containing the dyed biological particles and detecting information light therefrom, the system comprising:

• a path-defining structure of transparent material for defining a flow path through which liquid containing the dyed biological particles runs;
• an irradiation apparatus for irradiating light onto the particles passing through the flow path;
• a first detecting apparatus for detecting first information light obtained from the irradiated particle;
• and
• a second detecting apparatus for detecting second information light obtained from the irradiated particle;
• wherein said irradiation apparatus includes first optical elements for collecting light at a first position and second optical elements for collecting light at a second position, the first and second positions being spaced from each other by a predetermined distance along a central axis of the flow path;
• wherein at least one of said first and second detecting apparatuses includes first and second fiber optics, of which one ends are arranged adjacent the flow path so as to collect the information light from the particles irradiated at the first and second positions.

A system for collecting biological information on a dyed biological particle by irradiating light onto liquid containing the dyed biological particles and detecting information light therefrom, the system comprising:

• a path-defining structure of transparent material defining a flow path through which the liquid containing the dyed biological particles runs;
• an irradiation apparatus for irradiating light onto the particles passing through the flow path;
• a first detecting apparatus for detecting first information light obtained from the irradiated particle;
• a second detecting apparatus for detecting second information light obtained from the irradiated particle;
• wherein at least one of said first and second detecting apparatuses includes one or more fiber optics, of which one ends are arranged adjacent the flow oath so as to collect the information light;
• wherein at least one of said first and second detecting apparatus includes a spectrometer for splitting light components from light received by the fiber optics, and a plurality of optical detectors for reading information within the light split by the spectrometer;
• a signal processing apparatus;
• wherein the signal processing apparatus includes an A/W/H circuitry for calculating an area, width, and height of a plurality of analog signals output from a plurality of the optical detectors,
• an A/D converter for converting analog signals output from the A/W/H circuitry into digital signals,
• a first-in first-out memory for storing digital information based upon digital signals output the A/D converter and for simultaneously outputting a plurality of digital information,
• a compensation circuitry for compensating digital signals output from the memory, and
• a logarithmic calculator for logarithmically calculating digital information output from the compensation circuitry.

A system for collecting biological information on a dyed biological particle by irradiating light onto liquid containing the dyed biological particles and detecting information light therefrom, the system comprising:

• a path-defining structure of transparent material defining a flow path through which the liquid containing the dyed biological particles runs;
• an irradiation apparatus for irradiating light onto the particles passing through the flow path;
• a first detecting apparatus for detecting first information light obtained from the irradiated particle;
• a second detecting apparatus for detecting second information light obtained from the irradiated particle;
• wherein at least one of said first and second detecting apparatuses includes one or more fiber optics, of which one ends are arranged adjacent the flow path so as to collect the information light;
• a cylindrical container having a laminar-flow generating chamber provided upstream the flow path;
• a sheath-fluid line for supplying sheath fluid constituting a main body of the liquid from an upper end of the cylindrical container;
• a suspension-fluid tube arranged along the central axis of the cylindrical container for supplying suspension fluid containing the particles into the sheath fluid running within the laminar-flow generating chamber;
• a mechanism for adjusting a position of a suspension-fluid outlet of the suspension-fluid tube;
• and
• a drain line connected with a lower end of the laminar-flow generating chamber;
• a sheath-fluid source of the sheath fluid;
• a sheath-fluid waste of the sheath fluid;
• means for selectively connecting the drain line with either one of the sheath-fluid source and the sheath-fluid waste.

A system for collecting biological information on a dyed biological particle by irradiating light onto liquid containing the dyed biological particles and detecting information light therefrom, the system comprising:

• a path-defining structure of transparent material defining a flow path through which the liquid containing the dyed biological particles runs;
• an irradiation apparatus for irradiating light onto the particles passing through the flow path;
• a first detecting apparatus for detecting first information light obtained from the irradiated particle;
• a second detecting apparatus for detecting second information light obtained from the irradiated particle;
• wherein at least one of said first and second detecting apparatuses includes one or more fiber optics, of which one ends are arranged adjacent the flow path so as to collect the information light;
• charging means for charging a droplet injected from the flow path;
• a pair of deflecting plates provided adjacent a dropping path of the charged droplet;
• and
• a power source circuitry for generating an electrical field between a pair of the deflecting plates;
• wherein at least one of the deflecting plates is formed from a porous plate, and an aspirator being provided at rear end of the porous deflecting plate for aspirating droplet attached on the porous deflecting plate.