Insecticidal plant cyclotide with activity against Homopteran insects
AssigneeE. I. Du Pont de Nemours and Company
InventorsRafael Herrmann, Albert L. Lu, Billy F. McCutchen, James K. Presnail, Janet A. Rice, Clement W. K. Waine
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Abstract
The present invention relates to isolated nucleic acids encoding plant cyclotides. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
Claims10
A transformed plant cell having stably incorporated into its genome at least one DNA construct comprising an isolated nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence set forth in SEQ ID NO:
- 1, 4, or 5;
- and
- (b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2, 3, or 6;
- wherein said nucleotide sequence is operably linked to a promoter that drives expression in a plant cell.
A transformed plant having stably incorporated into its genome at least one DNA construct comprising an isolated nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence set forth in SEQ ID NO:
- 1, 4, or 5;
- and
- (b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2, 3, or 6;
- wherein said nucleotide sequence is operably linked to a promoter that drives expression in a plant cell.
A method for impacting a plant Homopteran pest comprising introducing into a plant or cell thereof at least one nucleotide construct comprising a nucleotide sequence operably linked to a promoter that drives expression of a coding sequence in plant cells, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence set forth in SEQ ID NO:
- 1, 4 or 5;
- and
- (b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2, 3 or 6.
Dependencies are inferred from the claim text.