Protein kinase stress-related proteins and methods of use in plants
AssigneeBASF Plant Science GmbH
InventorsDamian Allen, Ruoying Chen, Amber Shirley
Life of this patent
Estimating the term…
Abstract
A transgenic plant transformed by a Protein Kinase Stress-Related Protein (PKSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in the plant's increased growth under normal or stress conditions and/or increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated PKSRPs, and isolated nucleic acid coding PKSRPs, and vectors and host cells containing the latter.
Claims22
A transgenic plant cell transformed with an expression vector comprising an isolated polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence comprising nucleotides 1 to 1091 of SEQ ID NO:129;
- and
- b) a polynucleotide encoding a polypeptide having a sequence as set forth in amino acids 1 to 333 of SEQ ID NO:130.
A transgenic plant transformed with an expression cassette comprising an isolated polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence comprising nucleotides 1 to 1091 of SEQ ID NO:129;
- and
- b) a polynucleotide encoding a polypeptide having a sequence as set forth in amino acids 1 to 333 of SEQ ID NO:130.
A seed which is true breeding for a transgene comprising a polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence comprising nucleotides 1 to 1091 of SEQ ID NO:129;
- and
- b) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 333 of SEQ ID NO:130.
An isolated nucleic acid comprising a polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence comprising nucleotides 1 to 1091 of SEQ ID NO:129;
- and
- b) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 333 of SEQ ID NO:130.
A method of producing a drought-tolerant transgenic plant, the method comprising the steps of:
- a) transforming a plant cell with an expression vector comprising a polynucleotide selected from the group consisting of:
- i) a polynucleotide having a sequence comprising nucleotides 1 to 1091 of SEQ ID NO:129;
- and
- ii) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 333 of SEQ ID NO:130;
- b) regenerating transgenic plants from the transformed plant cell;
- c) assaying the regenerated plants for drought tolerance;
- and
- d) selecting regenerated plants that demonstrate improved drought tolerance as compared to a wild type variety of the plant.
Dependencies are inferred from the claim text.