Humanized anti-CCR2 antibodies and methods of use therefor
AssigneeMillennium Pharmaceuticals, Inc.
InventorsGregory J. LaRosa, Christopher Horvath, Walter Newman, S. Tarran Jones, Siobhan H. O'Brien, Theresa O'Keefe
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Abstract
The present invention relates to a humanized antibody or functional fragment thereof which binds to a mammalian (e.g., human) CC-chemokine receptor 2 (CCR2) or a portion of the receptor and blocks binding of a ligand to the receptor. The invention further relates to a method of inhibiting the interaction of a cell bearing mammalian CCR2 with a ligand thereof, and to use of the antibodies and fragments in therapeutic, prophylactic and diagnostic methods.
Claims38
An isolated nucleic acid molecule encoding a humanized immunoglobulin light chain or antigen-binding fragment thereof that binds to CC chemokine receptor 2 (CCR2) and comprises CDR1, CDR2 and CDR3 of the variable light chain of SEQ ID NO:9 and a human light chain framework region of the light chain of SEQ ID NO:11.
An isolated nucleic acid molecule encoding a humanized immunoglobulin heavy chain or antigen-binding fragment thereof that binds to CC chemokine receptor 2 (CCR2) and comprises CDR1, CDR2 and CDR3 of the variable heavy chain of SEQ ID NO:10 and a human heavy chain framework region of the heavy chain of SEQ ID NO:16.
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a humanized immunoglobulin light chain or antigen-binding fragment thereof that binds to CC chemokine receptor 2 (CCR2), said light chain or antigen-binding fragment thereof having an amino acid sequence comprising at least an antigen binding portion of the light chain variable region amino acid sequence of SEQ ID NO:
- 12, 13, 14, 15 or 107.
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a humanized immunoglobulin heavy chain or antigen-binding fragment thereof that binds to CC chemokine receptor 2 (CCR2), said heavy chain or antigen-binding fragment thereof having an amino acid sequence comprising at least an antigen binding portion of the heavy chain variable region amino acid sequence of SEQ ID NO:
- 17, 18, 19 or 20.
An isolated nucleic acid molecule encoding a humanized immunoglobulin light chain that binds to CC chemokine receptor 2 (CCR2) comprising:
- a) a first nucleic acid sequence encoding an antigen binding region comprising CDR1, CDR2 and CDR3 of the variable light chain of SEQ ID NO:9 and a human light chain framework region of the light chain of SEQ ID NO:11;
- and
- b) a second nucleic acid sequence encoding at least a portion of a constant region of an immunoglobulin of human origin.
An isolated nucleic acid molecule encoding a humanized immunoglobulin heavy chain that binds to CC chemokine receptor (CCR2) comprising:
- a) a first nucleic acid sequence encoding an antigen binding region comprising CDR1, CDR2 and CDR3 of the variable heavy chain of of SEQ ID NO:10 and a human heavy chain framework region of the heavy chain of SEQ ID NO:16;
- and
- b) a second nucleic acid sequence encoding at least a portion of a constant region of an immunoglobulin of human origin.
Dependencies are inferred from the claim text.